Secreted Expression of the Cap Gene of Porcine Circovirus Type 2 in Classical Swine Fever Virus C-Strain: Potential of C-Strain Used as a Vaccine Vector
文献类型: 外文期刊
第一作者: Zhang, Lingkai
作者: Zhang, Lingkai;Li, Yongfeng;Xie, Libao;Wang, Xiao;Sun, Yuan;Qiu, Hua-Ji;Gao, Xulei
作者机构:
关键词: classical swine fever virus;porcine circovirus type 2;C-strain;live virus vector;bivalent vaccine
期刊名称:VIRUSES-BASEL ( 影响因子:5.048; 五年影响因子:5.127 )
ISSN: 1999-4915
年卷期: 2017 年 9 卷 10 期
页码:
收录情况: SCI
摘要: Bivalent vaccines based on live attenuated viruses expressing a heterologous protein are an attractive strategy to address co-infections with various pathogens in the field. Considering the excellent efficacy and safety of the lapinized live attenuated vaccine C-strain (HCLV strain) of classical swine fever virus (CSFV), we proposed that C-strain has the potential as a viral vector for developing bivalent vaccines. To this end, we generated three recombinant viruses based on C-strain, one expressing the capsid (Cap) gene of porcine circovirus type 2 (PCV2) with the nuclear localization signal (NLS) (rHCLV-2ACap), and the other two expressing the PCV2 Cap gene without the NLS yet containing the signal peptide of the prolactin gene (rHCLV-pspCap) or that of the ubiquitin-specific peptidase gene (rHCLV-uspCap). All the recombinant viruses exhibited phenotypes similar to those of the parental virus and produced high-level anti-CSFV neutralizing antibodies (NAbs) in rabbits. Interestingly, rHCLV-uspCap and rHCLV-pspCap, but not rHCLV-2ACap, elicited detectable anti-Cap and -PCV2 NAbs in rabbits. Taken together, our data demonstrate that C-strain can be used as a viral vector to develop bivalent vaccines.
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