文献类型： 外文期刊

第一作者： Yuan, Guo-Liang

作者： Yuan, Guo-Liang;Li, Hong-Ju;Yang, Wei-Cai;Yuan, Guo-Liang

作者机构： Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol & Dev Biol, Beijing 100101, Peoples R China;Beijing Acad Agr & Forestry, Beijing Engn & Tech Res Ctr Hybrid Wheat, Beijing 100097, Peoples R China

期刊名称：SCIENTIFIC REPORTS （ 2020影响因子：4.38； 五年影响因子：5.134 ）

ISSN： 2045-2322

年卷期： 2017 年 7 卷

页码：

收录情况： SCI

摘要： Cells respond to many signals with a limited number of signaling components. Heterotrimeric G proteins and MAPK cascades are universally used by eukaryotic cells to transduce signals in various developmental processes or stress responses by activating different effectors. MAPK cascade is integrated with G proteins by scaffold protein during plant immunity. However, the molecular relationship between G proteins and MAPK modules in plant development is still unclear. In this study, we demonstrate that Arabidopsis G beta protein AGB1 interacts with MPK3 and 6, MKK4 and 5, as well as the regulatory domains of YODA (YDA), the upstream MEKK of MKK4/5. Remarkably, YDA interacts with the plasma membrane associated SHORT SUSPENSOR (SSP) through its N- and C-terminal region in vitro and in vivo. Additionally, genetic analysis shows that AGB1 functions together with MPK3/6 signaling cascade during the asymmetric division of the zygote. These data indicate that G beta may function likely as a scaffold, through direct physical interaction with the components of the MPK signaling module in plant development. Our results provide new insights into the molecular functions of G protein and will advance the understanding of the complex mechanism of kinase signaling cascades.

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