A new single-cell protein from Clostridium autoethanogenum as a functional protein for largemouth bass (Micropterus salmoides)

文献类型： 外文期刊

第一作者： Ma, Shifeng

作者： Ma, Shifeng;Liang, Xiaofang;Chen, Pei;Wang, Jie;Gu, Xu;Xue, Min;Qin, Yuchang;Ma, Shifeng;Blecker, Christophe

作者机构：

关键词： Clostridium autoethanogenum protein; Micropterus salmoides; Growth; Lipid metabolism; Hepatic and intestinal health; Gut-adherent microbiota

期刊名称：ANIMAL NUTRITION （ 影响因子：5.285； 五年影响因子：7.059 ）

ISSN： 2405-6383

年卷期： 2022 年 10 卷

页码：

收录情况： SCI

摘要： Clostridium autoethanogenum protein (CAP) is a new single-cell protein source originating from inactivated bacteria. An in vitro digestion experiment and an 8-wk growth experiment were conducted to evaluate the molecular weight distribution of the CAP hydrolysate, and the effects of dietary CAP levels on the growth performance, plasma parameters, hepatic and intestinal health, and the diversity of gutadherent microbiota of largemouth bass (Micropterus salmoides). The fish (initial body weight of 47.99 +/- 0.01 g) were fed diets where CAP gradually replaced 0% (CAPO), 12.5% (CAP12.5), 25% (CAP25), 37.5% (CAP37.5) and 50% (CAP50) of low-temperature steam dried anchovy fish meal (LTFM) in the diet. Results showed that the content of peptides below 1,000 Da in the CAP hydrolysate (0.56 mg/mL) was higher than that of the LTFM hydrolysate (0.48 mg/mL). Dietary CAP inclusion had no negative effect on growth performance, while whole-body lipid content significantly reduced in the CAP25 and CAP50 groups (P < 0.05). The plasma alanine aminotransferase activities and triglyceride concentrations in the CAP inclusion groups were significantly lower than those in the CAPO group (P < 0.05). The plasma aspartate aminotransferase activity was significantly reduced in the CAP37.5 group (P < 0.05). The richness and diversity of the gut-adhesive microbiota and the proportion of Clostridium sensu stricto 12 in the CAP50 group were significantly higher than those in the CAPO group (P < 0.05). Dietary CAP inclusion inhibited inflammatory responses by down-regulating the mRNA levels of interleukin 1 beta (IL1 beta), IL10 and transforming growth factor beta 1 (P < 0.05) in the liver. The mRNA levels of acetyl-CoA carboxylase 1 were significantly down-regulated in the CAP12.5, CAP25 and CAP37.5 groups (P < 0.05), while that of fatty acid synthase was significantly down-regulated in the CAP50 group (P < 0.05). These results demonstrate that dietary CAP inclusion could improve the hepatic and intestinal health of largemouth bass, and can be helpful to further develop CAP as a functional feed ingredient. (C) 2022 Chinese Association of Animal Science and Veterinary Medicine. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.

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