Identification of candidate genes regulating seed oil content by QTL mapping and transcriptome sequencing in Brassica napus

文献类型： 外文期刊

第一作者： Xiao, Zhongchun

作者： Xiao, Zhongchun;Zhang, Chao;Qu, Cunmin;Wei, Lijuan;Zhang, Liyuan;Yang, Bo;Lu, Kun;Li, Jiana

作者机构：

关键词： Brassica napus; seed oil content; QTL mapping; differentially expressed genes (DEGs); candidate genes

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Increasing oil production is a major goal in rapeseed (Brassica napus) molecular breeding programs. Identifying seed oil content (SOC)-related candidate genes is an important step towards achieving this goal. We performed quantitative trait locus (QTL) mapping of SOC in B. napus using a high-density SNP genetic map constructed from recombinant inbred lines and the Illumina Infinium (TM) 60K SNP array. A total of 26 QTLs were detected in three years on A01, A03, A05, A06, A09, C01, CO3 and C05, which accounted for 3.69%similar to 18.47% of the phenotypic variation in SOC. Of these, 13 QTLs are reported here for the first time. 1713 candidate genes in the 26 QTLs confidence interval were obtained. We then identified differentially expressed genes (DEGs) between the high- and low-SOC accessions, to narrow down our focus to 21 candidate genes (Y1-Y21) related to SOC, and we will focus on 11 (Y1-Y11) candidate genes that contribute to the formation of high-SOC. In addition to providing insight into the genetic basis of SOC in B. napus, the loci identified and candidate genes in this study can be used in molecular breeding strategies to increase SOC in this important seed crop.

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