SGS3 Cooperates with RDR6 in Triggering Geminivirus-Induced Gene Silencing and in Suppressing Geminivirus Infection in Nicotiana Benthamiana

文献类型： 外文期刊

第一作者： Zhou, Xueping

作者： Zhou, Xueping;Li, Fangfang;Wang, Yaqin;Zhou, Xueping

作者机构：

关键词： RNA silencing;geminivirus;RDR6;SGS3;resistance

期刊名称：VIRUSES-BASEL （ 影响因子：5.048； 五年影响因子：5.127 ）

ISSN： 1999-4915

年卷期： 2017 年 9 卷 9 期

页码：

收录情况： SCI

摘要： RNA silencing has an important role in defending against virus infection in plants. Plants with the deficiency of RNA silencing components often show enhanced susceptibility to viral infections. RNA-dependent RNA polymerase (RDRs) mediated-antiviral defense has a pivotal role in resistance to many plant viruses. In RDR6-mediated defense against viral infection, a plant-specific RNA binding protein, Suppressor of Gene Silencing 3 (SGS3), was also found to fight against some viruses in Arabidopsis. In this study, we showed that SGS3 from Nicotiana benthamiana (NbSGS3) is required for sense-RNA induced post-transcriptional gene silencing (S-PTGS) and initiating sense-RNA-triggered systemic silencing. Further, the deficiency of NbSGS3 inhibited geminivirus-induced endogenous gene silencing (GIEGS) and promoted geminivirus infection. During TRV-mediated NbSGS3 or N. benthamiana RDR6 (NbRDR6) silencing process, we found that their expression can be effectively fine-tuned. Plants with the knock-down of both NbSGS3 and NbRDR6 almost totally blocked GIEGS, and were more susceptible to geminivirus infection. These data suggest that NbSGS3 cooperates with NbRDR6 against GIEGS and geminivirus infection in N. benthamiana, which provides valuable information for breeding geminivirus-resistant plants.

分类号：

  • 相关文献

[1]CHINESE SQUASH LEAF CURL VIRUS - A NEW WHITEFLY-TRANSMITTED GEMINIVIRUS. WANG, XF,TIAN, B,CAI, JH. 1995

[2]Genomic Analysis of the Natural Population of Wheat dwarf virus in Wheat from China and Hungary. Liu Yan,Wang Biao,Wu Bei-lei,Wang Xi-feng,Liu Yan,Wu Yuan-hua,Vida, Gyula,Cseplo-Karolyi, Monika. 2012

[3]The Cap Snatching of Segmented Negative Sense RNA Viruses as a Tool to Map the Transcription Start Sites of Heterologous Co-infecting Viruses. Lin, Wenzhong,Qiu, Ping,Jin, Jing,Liu, Shunmin,Ul Islam, Saif,Zhang, Jie,Du, Zhenguo,Wu, Zujian,Yang, Jinguang,Kormelink, Richard,Du, Zhenguo,Wu, Zujian. 2017

[4]Tobacco RING E3 Ligase NtRFP1 Mediates Ubiquitination and Proteasomal Degradation of a Geminivirus-Encoded beta C1. Hu, Tao,Bao, Min,Cao, Linge,Song, Fengmin,Zhou, Xueping,Bao, Min,Zhou, Xueping,Zhang, Huawei,Xie, Qi,Shen, Qingtang. 2016

[5]Mimic Phosphorylation of a beta C1 Protein Encoded by TYLCCNB Impairs Its Functions as a Viral Suppressor of RNA Silencing and a Symptom Determinant. Zhong, Xueting,Wang, Zhan Qi,Xiao, Ruyuan,Cao, Linge,Wang, Yaqin,Xie, Yan,Zhou, Xueping,Zhou, Xueping.

[6]The AC5 protein encoded by Mungbean yellow mosaic India virus is a pathogenicity determinant that suppresses RNA silencing-based antiviral defenses. Xu, Xiongbiao,Huang, Changjun,Gu, Zhouhang,Cao, Linge,Hu, Tao,Li, Zhenghe,Zhou, Xueping,Li, Fangfang,Zhou, Xueping,Ding, Ming.

[7]Geminiviruses and their application in biotechnology. Ding Bo,Zhou Xue-ping. 2017

[8]Tomato Yellow Leaf Curl Virus V2 Interacts with Host Histone Deacetylase 6 To Suppress Methylation-Mediated Transcriptional Gene Silencing in Plants. Wang, Bi,Wang, Yaqin,Xie, Yan,Zhou, Xueping,Wang, Bi,Yang, Xiuling,Zhou, Xueping. 2018

[9]Knock down of chitosanase expression in phytopathogenic fungus Fusarium solani and its effect on pathogenicity. Zhang, Bo,Li, Changsong,Liu, Huaiwei,Bao, Xiaoming. 2010

[10]Generation of double-virus-resistant marker-free transgenic potato plants. Bai, Yunfeng,Guo, Zhihua,Wang, Xiaoqi,Bai, Dongmei,Zhang, Weifeng. 2009

[11]Improved Pathogenicity of a Beet Black Scorch Virus Variant by Low Temperature and Co-infection with its Satellite RNA. Xu, Jin,Liu, Deshui,Zhang, Yongliang,Wang, Ying,Han, Chenggui,Li, Dawei,Yu, Jia-Lin,Wang, Xian-Bing,Xu, Jin. 2016

[12]Genome-wide identification of Dicer-like, Argonaute and RNA-dependent RNA polymerase gene families and their expression analyses in response to viral infection and abiotic stresses in Solanum lycopersicum. Mao, Zhen-Chuan,Kang, Hou-Xiang,Chen, Guo-Hua,Yang, Yu-Hong,Xie, Bing-Yan,Bai, Miao,Yang, Guo-Shun,Chen, Wen-Ting.

[13]Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Inhibits RNA-Mediated Gene Silencing by Targeting Ago-2. Chen, Jing,Zhang, Gaiping,Chen, Jing,Shi, Xibao,Wang, Li,Luo, Jun,Xing, Guangxu,Deng, Ruiguang,Zhang, Gaiping,Shi, Xibao,Zhang, Xiaozhuan,Yang, Hong,Li, Jinting,Wang, Aiping,Zhang, Gaiping. 2015

[14]High-Level Accumulation of Exogenous Small RNAs Not Affecting Endogenous Small RNA Biogenesis and Function in Plants. Shen Wan-xia,Zhou Chang-yong,Shen Wan-xia,Smith, Neil A.,Wang Ming-bo,Shen Wan-xia,Zhou Chang-yong. 2014

[15]Durable field resistance to wheat yellow mosaic virus in transgenic wheat containing the antisense virus polymerase gene. Chen, Ming,Ma, Youzhi,Du, Lipu,Ye, Xingguo,Pang, Junlan,Zhang, Xinmei,Li, Liancheng,Xu, Huijun,Sun, Liying,Chen, Jiong,Andika, Ida B.,Chen, Jianping.

[16]A novel strategy to enhance resistance to Cucumber mosaic virus in tomato by grafting to transgenic rootstocks. Bai Miao,Chen Wen-ting,Yang Gun-shun,Xie Bing-yan. 2016

[17]CMV2b-AGO Interaction Is Required for the Suppression of RDR-Dependent Antiviral Silencing in Arabidopsis. Fang, Yuan-Yuan,Zhao, Jian-Hua,Liu, Shang-Wu,Wang, Sheng,Duan, Cheng-Guo,Guo, Hui-Shan,Liu, Shang-Wu,Guo, Hui-Shan,Duan, Cheng-Guo. 2016

[18]Silencing of NbXrn4 facilitates the systemic infection of Tobacco mosaic virus in Nicotiana benthamiana. Yan, Fei,Lu, Yuwen,Lin, Lin,Zheng, Hongying,Chen, Jianping,Yan, Fei,Lu, Yuwen,Lin, Lin,Zheng, Hongying,Chen, Jianping,Peng, Jiejun,Chen, Hairu,Yang, Jian,Jiang, Shanshan. 2011

[19]Characterization of small interfering RNAs derived from Rice black streaked dwarf virus in infected maize plants by deep sequencing. Li, Mingjun,Li, Yongqiang,Xia, Zihao,Zhou, Tao,Fan, Zaifeng,Li, Mingjun,Li, Yongqiang,Xia, Zihao,Zhou, Tao,Fan, Zaifeng,Di, Dianping,Zhang, Aihong,Miao, Hongqin.

[20]RNA-Dependent RNA Polymerase 1 from Nicotiana tabacum Suppresses RNA Silencing and Enhances Viral Infection in Nicotiana benthamiana. Ying, Xiao-Bao,Dong, Li,Zhu, Hui,Duan, Cheng-Guo,Du, Quan-Sheng,Lv, Dian-Qiu,Fang, Yuan-Yuan,Fang, Rong-Xiang,Guo, Hui-Shan,Ying, Xiao-Bao,Dong, Li,Zhu, Hui,Duan, Cheng-Guo,Du, Quan-Sheng,Lv, Dian-Qiu,Fang, Yuan-Yuan,Fang, Rong-Xiang,Guo, Hui-Shan,Ying, Xiao-Bao,Zhu, Hui,Lv, Dian-Qiu,Antonio Garcia, Juan.

作者其他论文 更多>>

微信小程序