FOXA transcriptional factor modulates insect susceptibility to Bacillus thuringiensis Cry1Ac toxin by regulating the expression of toxin-receptor ABCC2 and ABCC3 genes

文献类型： 外文期刊

第一作者： Li, Jianghuai

作者： Li, Jianghuai;Ma, Yuemin;Yuan, Wanli;Wang, Jia;Peng, Jianxin;Peng, Rong;Yang, Yongbo;Liu, Kaiyu;Xiao, Yutao;Liu, Chenxi;Soberon, Mario;Bravo, Alejandra

作者机构：

关键词： Bacillus thuringiensis;Toxin receptor;Transcription factor;ATP binding cassette;FOXA

期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.714； 五年影响因子：4.953 ）

ISSN： 0965-1748

年卷期： 2017 年 88 卷

页码：

收录情况： SCI

摘要： Cry toxins produced by Bacillus thuringiensis (Bt) are insecticidal proteins widely used in insect control. Recently, it was shown that ATP-binding cassette transporter proteins (ABC) such as ABCC2, ABCC3, ABCG1 and ABCA2 are implicated in the insecticidal action of Cry toxins as putative receptors. However, the transcriptional regulators involved in the expression of ABC transporter genes remain unknown. Sequence analysis of promoter regions of ABCC2 gene from Helicoverpa armigera and ABCC3 gene from Spodoptera litura Sl-HP cultured cells, revealed the potential participation of Forkhead box protein A (FOXA), a transcription factor that regulates the expression of genes through remodeling chromatin. To determine if FOXA was involved in regulating expression of ABCC2 and ABCC3 genes, the expression of FOXA, ABCC2 and ABCC3 was compared in SI-HP cells that are sensitive to CrylAc toxin with those in S. frugiperda Sf9 cells that are not sensitive to the toxin. Expression levels of those genes were significantly higher in SI-HP than in Sf9 cells. Transient expression of FOXA in Sf9 cells activated ABCC2 and ABCC3 transcription, which directly correlated with enhanced CrylAc-susceptibility in these cells. Silencing of FOXA gene expression by RNAi in H. armigera larvae resulted in a decreased expression of ABCC2 and ABCC3 without affecting expression of other Cry toxin receptor genes such as alkaline phosphatase, aminopeptidase or cadherin. Silencing of FOXA gene expression also resulted in a CrylAc-tolerant phenotype since lower mortality and higher pupation rate were observed in diet containing CrylAc protoxin in comparison with the control group. These results demonstrate that FOXA up-regulates expression of the CrylAc-toxin receptor ABCC2 and ABCC3 genes, and that lower FOXA expression correlates with tolerance to Cry toxin in cell lines and in lepidopteran larvae. (C) 2017 Elsevier Ltd. All rights reserved.

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