ZYGOTE-ARREST 3 that encodes the tRNA ligase is essential for zygote division in Arabidopsis

文献类型： 外文期刊

第一作者： Yang, Ke-Jin

作者： Yang, Ke-Jin;Guo, Lei;Hou, Xiu-Li;Gong, Hua-Qin;Liu, Chun-Ming;Yang, Ke-Jin;Guo, Lei;Hou, Xiu-Li;Yang, Ke-Jin;Liu, Chun-Ming

作者机构：

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）

ISSN： 1672-9072

年卷期： 2017 年 59 卷 9 期

页码：

收录情况： SCI

摘要： In sexual organisms, division of the zygote initiates a new life cycle. Although several genes involved in zygote division are known in plants, how the zygote is activated to start embryogenesis has remained elusive. Here, we showed that a mutation in ZYGOTE-ARREST 3 (ZYG3) in Arabidopsis led to a tight zygote-lethal phenotype. Map-based cloning revealed that ZYG3 encodes the transfer RNA (tRNA) ligase AtRNL, which is a single-copy gene in the Arabidopsis genome. Expression analyses showed that AtRNL is expressed throughout zygotic embryogenesis, and in meristematic tissues. Using pAtRNL::cAtRNL-sYFP-complemented zyg3/zyg3 plants, we showed that AtRNL is localized exclusively in the cytoplasm, suggesting that tRNA splicing occurs primarily in the cytoplasm. Analyses using partially rescued embryos showed that mutation in AtRNL compromised splicing of intron-containing tRNA. Mutations of two tRNA endonuclease genes, SEN1 and SEN2, also led to a zygote-lethal phenotype. These results together suggest that tRNA splicing is critical for initiating zygote division in Arabidopsis.

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