A novel low-temperature active alkaline pectate lyase from Klebsiella sp Y1 with potential in textile industry

文献类型： 外文期刊

第一作者： Yuan, Peng

作者： Yuan, Peng;Meng, Kun;Luo, Huiying;Shi, Pengjun;Huang, Huoqing;Bai, Yingguo;Yang, Peilong;Yao, Bin

作者机构：

关键词： Klebsiella sp Y1;Alkaline pectate lyase;Escherichia coli;Bioscouring

期刊名称：PROCESS BIOCHEMISTRY （ 影响因子：3.757； 五年影响因子：3.665 ）

ISSN： 1359-5113

年卷期： 2011 年 46 卷 10 期

页码：

收录情况： SCI

摘要： Alkaline pectate lyases are favorable for the textile industry. Here we report the cloning of a pectate lyase gene (pl A), from Klebsiella sp. Y1, and its heterologous expression in Escherichia coli. The full-length pl A consists of 1710 bp and encodes for a 569-amino acid polypeptide including a putative 22-residue signal peptide and a catalytic domain belonging to pectate lyase family 2. The recombinant enzyme (r-PL A) was purified to electrophoretic homogeneity by single-step Ni2+-NTA affinity chromatography and showed an apparent molecular weight of similar to 60 kDa. The pH and temperature optima of r-PL A were found to be 9.0 and 30-50 degrees C, respectively. r-PL A was highly active at low temperatures, exhibiting >60% of the maximal activity at 20 degrees C and >20% activity even at 0 degrees C. The enzyme was stable in a broad alkaline pH range of 7.0-12.0 for 1 h at 37 degrees C. The values of K-m(app) and V-max(app) of r-PL A for polygalacturonic acid were 2.47 mg/ml and 11.94 mu mol/min/mg, respectively. Compared with the commercial compound pectinase from Novozymes, purified r-PL A showed similar efficacy in reducing the intrinsic viscosity of polygalacturonic acid (68.8% vs. 67.1%) and in bioscouring of jute (7.38% vs. 7.58%). Thus r-PL A is a valuable material for the textile industry. (C) 2011 Elsevier Ltd. All rights reserved.

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