Tissue distribution and expression of signaling lymphocyte activation molecule receptor to peste des petits ruminant virus in goats detected by real-time PCR
文献类型: 外文期刊
第一作者: Meng, Xuelian
作者: Meng, Xuelian;Dou, Yongxi;Zhai, Junjun;Zhang, Hairui;Yan, Fengchao;Shi, Xiaoni;Luo, Xuelong;Li, Hui;Cai, Xuepeng
作者机构:
关键词: Real-time RT-PCR;SLAM receptor;Expression;Tissue distribution;Goat
期刊名称:JOURNAL OF MOLECULAR HISTOLOGY ( 影响因子:2.611; 五年影响因子:2.502 )
ISSN: 1567-2379
年卷期: 2011 年 42 卷 5 期
页码:
收录情况: SCI
摘要: In the present study, we investigated the tissue distribution and expression of signaling lymphocyte activation molecule (SLAM) in 40 tissues and organs of goats by real-time RT-PCR, in order to determine the role of these receptors in tissue tropism. SLAM mRNA was detected in all the samples investigated. The expression of SLAM mRNA was detected at high levels in spleen, mesenteric lymph node, hilar lymph node, mandibular lymph node, superficial cervical lymph node, nasal mucosa, duodenum, heart, gallbladder, thymus and blood; this is similar to the tissue tropism of peste des petits ruminant virus. However, it was surprising that expression of SLAM was low in lungs, colon and rectum which are the major sites of replication of PPRV. In addition, very low levels were detected in larynx, tongue and esophagus, which suggest the possible presence of an alternative receptor for PPRV. This study provided the first data on caprine SLAM for use in further studies of the pathogenesis of PPRV in goats.
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