Improved secretory production of calf prochymosin by codon optimization and disruption of PMR1 in Kluyveromyces lactis GG799

文献类型: 外文期刊

第一作者: Feng, Zhen

作者: Feng, Zhen;Ren, Jing;Zhang, Yanhe;Hu, Sen

作者机构:

关键词: Kluyveromyces lactis;codon optimization;PMR1;prochymosin

期刊名称:AFRICAN JOURNAL OF BIOTECHNOLOGY ( 影响因子:0.573; 五年影响因子:0.794 )

ISSN: 1684-5315

年卷期: 2011 年 10 卷 52 期

页码:

收录情况: SCI

摘要: Chymosin as an important industrial enzyme used widely in cheese manufacture. In our preliminary study, low yields (80 U mL(-1)) were obtained when Kluyveromyces lactis GG799 was used to express chymosin. We investigated whether this poor secretion could be improved by codon optimization and disruption of PMR1 gene in K. lactis GG799. Our study shows that combination of codon optimization and disruption of the PMR1 gene resulted in secretion level of chymosin that was increased 10-fold. Our results show that codon optimization and disruption of the PMR1 gene synergistically stimulate the secretion of calf prochymosin in K. lactis GG799.

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