Generation of an infectious clone of HuN4-F112, an attenuated live vaccine strain of porcine reproductive and respiratory syndrome virus
文献类型: 外文期刊
第一作者: Zhang, Shanrui
作者: Zhang, Shanrui;Zhou, Yanjun;Jiang, Yifeng;Li, Guoxin;Yan, Liping;Yu, Hai;Tong, Guangzhi
作者机构:
关键词: Infectious clone;PRRSV;HuN4-F112
期刊名称:VIROLOGY JOURNAL ( 影响因子:4.099; 五年影响因子:3.719 )
ISSN: 1743-422X
年卷期: 2011 年 8 卷
页码:
收录情况: SCI
摘要: Background: Nowadays, PRRS has become one of the most economically important infectious diseases of pig worldwide. To better characterize and understand the molecular basis of PRRSV virulence determinants, it would be important to develop the infectious cDNA clones. In this regard, HuN4-F112, a live-attenuated North-American-type PRRSV vaccine strain, could serve as an excellent model. Results: In the study, genomic sequence of HuN4-F112, an attenuated vaccine virus derived from the highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) HuN4 strain, was determined and its full-length cDNA was cloned. Capped RNA was transcribed in vitro from the cDNA clone and transfected into BHK-21 cells. The supernatant from transfected monolayers were serially passaged in Marc-145 cells. The rescued virus exhibited a similar growth pattern to its parental virus in Marc-145 cells with peak titers at 48 h post-infection. Conclusion: In conclusion, we rescued virus from an infectious cDNA clone of attenuated vaccine. It is possible in the future that a new attenuated PRRSV vaccine with broader specificity and good immunogenicity can be designed in vitro via an infectious cDNA clone platform coupled with validated information on virulence determinants.
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