Troglitazone Induced Apoptosis via PPAR gamma Activated POX-induced ROS Formation in HT29 Cells

文献类型： 外文期刊

第一作者： Wang Jing

作者： Wang Jing;Du YuGuo;Lv XiaoWen;Shi JiePing;Hu XiaoSong

作者机构：

关键词： Troglitazone;Apoptosis;HT29;POX;ROS formation;PPAR gamma;Cytochrome c release

期刊名称：BIOMEDICAL AND ENVIRONMENTAL SCIENCES （ 影响因子：3.118； 五年影响因子：3.219 ）

ISSN： 0895-3988

年卷期： 2011 年 24 卷 4 期

页码：

收录情况： SCI

摘要： Objective In order to investigate the potential mechanisms in troglitazone-induced apoptosis in HT29 cells, the effects of PPAR gamma and POX-induced ROS were explored. Methods [3- (4, 5)-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MU) assay, Annexin V and PI staining using FACS, plasmid transfection, ROS formation detected by DCFH staining, RNA interference, RT-PCR & RT-QPCR, and Western blotting analyses were employed to investigate the apoptotic effect of troglitazone and the potential role of PPAR gamma pathway and POX-induced ROS formation in HT29 cells. Results Troglitazone was found to inhibit the growth of HT29 cells by induction of apoptosis. During this process, mitochondria related pathways including ROS formation, POX expression and cytochrome c release increased, which were inhibited by pretreatment with GW9662, a specific antagonist of PPAR gamma. These results illustrated that POX upregulation and ROS formation in apoptosis induced by troglitazone was modulated in PPAR gamma-dependent pattern. Furthermore, the inhibition of ROS and apoptosis after POX siRNA used in troglitazone-treated HT29 cells indicated that POX be essential in the ROS formation and PPAR gamma-dependent apoptosis induced by troglitazone. Conclusion The findings from this study showed that troglitazone-induced apoptosis was mediated by POX-induced ROS formation, at least partly, via PPAR gamma activation.

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