Differential expression of microRNA-2b with potential target coding P25 in the fifth instar larvae posterior silk gland of the silkworm

文献类型: 外文期刊

第一作者: Huang, Yong

作者: Huang, Yong;Shen, Xing Jia;Tang, Shun Ming;Wang, Sheng Peng;Zhao, Qiao Ling;Zou, Quan;Huang, Yong;Shen, Xing Jia;Tang, Shun Ming;Wang, Sheng Peng;Zhao, Qiao Ling

作者机构:

关键词: microRNA;silkworm;in silico;P25 gene

期刊名称:MOLECULAR BIOLOGY ( 影响因子:1.374; 五年影响因子:1.266 )

ISSN: 0026-8933

年卷期: 2011 年 45 卷 4 期

页码:

收录情况: SCI

摘要: MicroRNAs (miRNAs) are a class of non-protein coding small RNAs that regulate a gene expression at the post-transcriptional level. Using in silico screening, we found that the 3'-untranslated regions of the P25 gene mRNA are perfectly complementary to nucleotides 2-8 at the 5' end of the miRNA-2b (miR-2b). The expression of miR-2b and the P25 gene in posterior silk gland of the fifth instar larval silkworm was investigated using real-time PCR detection method. The results indicated that expression of the P25 gene was very high in the posterior silk gland during the fifth instar larvae, whereas a level of miR-2b sharply decreased until reaching the lowest one on the 8th day. The expression patterns of miR-2b and P25 gene indicate that miR-2b might act as a fine-tuning regulator of expression of the P25 gene at the post-transcriptional level.

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