N-(2-Chloro-4-pyridyl)-N'-phenylurea promotes microspore embryogenesis and plant regeneration in pakchoi (Brassica rapa L. ssp. chinensis)

文献类型： 外文期刊

第一作者： Li, Xue

作者： Li, Xue;Bai, Yuanzhi;Wang, Nan;Feng, Hui;Li, Xue;Bai, Yuanzhi;Wang, Nan;Feng, Hui;Huang, Zhiyin;Fan, Weiqiang

作者机构：

关键词： Pakchoi; CPPU; Isolated microspore culture; Embryogenesis

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.4； 五年影响因子：2.6 ）

ISSN： 0167-6857

年卷期： 2025 年 161 卷 2 期

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收录情况： SCI

摘要： Hybrids frequently outperform traditional varieties due to their enhanced yield and robust stress tolerance. A critical step in hybrid seed production is the development of homozygous inbred lines. To expedite the breeding process, we employed the isolated microspore culture (IMC) technique to obtain doubled haploid (DH) lines, but the rate of some microspore embryogenesis was extremely low. This study was aimed at establishing an effective microspore culture program for recalcitrant genotypes of pakchoi. We selected N-(2-Chloro-4-pyridyl)-N'-phenyl urea (CPPU) as an inducer for microspore embryogenesis since it was reported that CPPU could promote plant cell division. Three pakchoi lines 21Q04, 21Q08 and 21Q10 were used to test the CPPU effects. Results indicated that the most efficient CPPU concentration to enhance microspore embryogenesis was 0.01 mg/L; and at that concentration, the embryo yields of the three genotypes were 5.54, 3.22 and 2.43 times higher than those of control, respectively. The frequency of direct seedling formation was promoted by changing the inoculation direction of embryos in MS solid medium. These measures facilitate the application of microspore cultivation in pakchoi breeding.

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