A hevein-like protein and a class I chitinase with antifungal activity from leaves of the paper mulberry

文献类型： 外文期刊

第一作者： Pan, Ying-Hong

作者： Pan, Ying-Hong;Zhao, Ming;Ma, Yan;Zhang, Chun-Hua;Yuan, Wen-Xia

作者机构：

关键词： antifungal protein;fast protein liquid chromatography;matrix-assisted laser desorption ionization time-of-flight mass;spectrometry;peptide mapping fingerprint analysis

期刊名称：BIOMEDICAL CHROMATOGRAPHY （ 影响因子：1.902； 五年影响因子：1.809 ）

ISSN： 0269-3879

年卷期： 2011 年 25 卷 8 期

页码：

收录情况： SCI

摘要： Paper mulberry (Broussonetia papyrifera, syn. Morus papyrifera L.) is a Chinese traditional medicine and its low-molecular-weight extracts are reported to have antifungal activity. In this study, two proteins (PMAPI and PMAPII) with activity against Trichoderma viride were obtained from paper mulberry leaves with a fast protein liquid chromatography (FPLC) unit. The purification protocol employed (NH(4))(2)SO(4) precipitation, ion-exchange chromatography and hydrophobic-interaction chromatography on FPLC. Molecular masses were 18,798 Da for PMAPI, and 31,178 Da for PMAPII determined by Matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Peptide mapping fingerprint analysis showed that PMAPI has no peptides similar to PMAPII. N-terminal amino acid sequencing revealed that PMAPI is a hevein-like protein, and PMAPII is a class I chitinase. They both had a half-maximal inhibitory concentration (IC50) of 0.1 mu g/mu L against T. viride. This is the first report of high-molecular-weight extracts with antifungal activity from paper mulberry. Copyright (C) 2011 John Wiley & Sons, Ltd.

分类号：

  • 相关文献

[1]Purification and characterization of a protein with antifungal, antiproliferative, and HIV-1 reverse transcriptase inhibitory activities from small brown-eyed cowpea seeds. Tian, Guo-Ting,Zhu, Meng Juan,Wu, Ying Ying,Liu, Qin,Wang, He Xiang,Zhu, Meng Juan,Wu, Ying Ying,Liu, Qin,Wang, He Xiang,Ng, Tzi Bun. 2013

[2]Bacisubin, an antifungal protein with ribonuclease and hemagglutinating activities from Bacillus subtilis strain B-916. Chen, Zhiyi,Ng, T. B.,Zhang, Jie,Zhou, Mingguo,Song, Fuping,Lu, Fan,Liu, Youzhou. 2007

[3]Primary purification of two antifungal proteins from leaves of the fig (Ficus carica L.). Pan, Ying-hong,Zhao, Ming,Ma, Yan,Yuan, Wen-xia,Yan, Wei. 2011

[4]Cloning, characterization, and antifungal activity of an endo-1,3-beta-d-glucanase from Streptomyces sp S27. Shi, Pengjun,Yao, Guoyu,Yang, Peilong,Li, Ning,Luo, Huiying,Bai, Yingguo,Wang, Yaru,Yao, Bin,Yao, Guoyu. 2010

[5]Lipopeptides, a novel protein, and volatile compounds contribute to the antifungal activity of the biocontrol agent Bacillus atrophaeus CAB-1. Zhang, Xiaoyun,Li, Baoqing,Guo, Qinggang,Lu, Xiuyun,Li, Shezeng,Ma, Ping,Wang, Ye.

[6]Isolation and characterization of an antifungal protein from Bacillus licheniformis HS10. Wang, Zhixin,Zheng, Li,Yang, Xiaona,Liu, Hongxia,Guo, Jianhua,Wang, Zhixin,Zheng, Li,Yang, Xiaona,Liu, Hongxia,Guo, Jianhua,Wang, Zhixin,Zheng, Li,Yang, Xiaona,Liu, Hongxia,Guo, Jianhua,Wang, Yunpeng,Zheng, Li,Zheng, Li.

[7]Molecular cloning and characterization of a mannose-binding lectin gene from Crinum asiaticum. Pang, YZ,Liao, ZH,Zhang, L,Sun, XF,Lu, YQ,Wang, SG,Tang, KX.

[8]Qualitative Analysis of Unknown Color Additive by Liquid Chromatography-Ion Trap-Time of Flight Tandem Mass Spectrometry. Lei Shao-Rong,Guo Ling-An,Ji Feng. 2013

[9]Development of "one-pot" method for multi-class compounds in porcine formula feed by multi-function impurity adsorption cleaning followed ultra-performance liquid chromatography-tandem mass spectrometry detection. Wang, Peilong,Wang, Xiao,Zhang, Wei,Su, Xiaoou,Wang, Peilong,Wang, Xiao,Zhang, Wei,Su, Xiaoou.

[10]Generic DART-MS platform for monitoring the on-demand continuous-flow production of pharmaceuticals: Advancing the quantitative protocol for caffeates in microfluidic biocatalysis. Xu, Yan,Zhang, Dong-Yang,Liu, Xi,Sheng, Sheng,Wu, Guo-Hua,Wang, Jun,Wu, Fu-An,Xu, Yan,Zhang, Dong-Yang,Sheng, Sheng,Wu, Guo-Hua,Wang, Jun,Wu, Fu-An,Meng, Xiang-Yun.

[11]Low temperature purification method for the determination of abamectin and ivermectin in edible oils by liquid chromatography-tandem mass spectrometry. Huang, Jian-Xiang,Lu, Da-Hai,Wan, Kai,Wang, Fu-Hua. 2014

[12]Simultaneous determination of three pesticide adjuvant residues in plant-derived agro-products using liquid chromatography-tandem mass spectrometry. Li, Hui,Jiang, Zejun,Cao, Xiaolin,Su, Hang,Shao, Hua,Jin, Fen,Wang, Jing,Abd El-Aty, A. M.,Abd El-Aty, A. M.. 2017

[13]Hydrophilic interaction chromatography/tandem mass spectrometry for the determination of melamine in royal jelly and royal jelly lyophilized powder. Zhou, Jinhui,Zhao, Jing,Xue, Xiaofeng,Zhang, Jinzhen,Chen, Fang,Li, Yi,Wu, Liming,Li, Chenchen,Mi, Jianqiu.

[14]Quantitative determination of 26 steroids in eggs from various species using liquid chromatography-triple quadrupole-mass spectrometry. Mi, Xiaoxia,Li, Sicong,Li, Yanhua,Wang, Kaigiang,Zhu, Dan,Chen, Gang.

[15]Enantioselective determination of triazole fungicide simeconazole in vegetables, fruits, and cereals using modified QuEChERS (quick, easy, cheap, effective, rugged and safe) coupled to gas chromatography/tandem mass spectrometry. Dong, Fengshou,Xu, Jun,Liu, Xingang,Li, Yuanbo,Zheng, Yongquan,Shan, Weili.

[16]Research on the relationship between phenolic acids and rooting of tree peony (Paeonia suffruticosa) plantlets in vitro. Shang, Wenqian,Wang, Zheng,He, Songlin,He, Dan,Liu, Yiping,He, Songlin,Fu, Zhenzhu.

作者其他论文 更多>>

微信小程序