The discovery approaches and detection methods of microRNAs

文献类型: 外文期刊

第一作者: Huang, Yong

作者: Huang, Yong;Wang, Sheng Peng;Tang, Shun Ming;Zhang, Guo Zheng;Shen, Xing Jia;Huang, Yong;Wang, Sheng Peng;Tang, Shun Ming;Zhang, Guo Zheng;Shen, Xing Jia;Zou, Quan

作者机构:

关键词: MicroRNAs;Computational methods;Experimental detection

期刊名称:MOLECULAR BIOLOGY REPORTS ( 影响因子:2.316; 五年影响因子:2.357 )

ISSN: 0301-4851

年卷期: 2011 年 38 卷 6 期

页码:

收录情况: SCI

摘要: MicroRNAs (miRNAs) are small, highly conserved, non-coding RNAs that regulate gene expression of target mRNAs through cleavage or translational inhibition. Computer-based approaches for miRNA gene identification are being considered as indispensable in miRNAs research. Similarly, experimental approaches for detection of miRNAs are crucial to the testing and validating of computational algorithms. The detection of miRNAs in tissues or cells can supply valuable information for investigating the biological function of these molecules. Selective and highly sensitive detection methods will pave the way for extended understanding of miRNA function within organisms. In this review, we summarize the various computational methods for identification of miRNAs as well as the methodologies that have been developed to detection miRNAs.

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