Development of a tailored vaccine against challenge with very virulent infectious bursal disease virus of chickens using reverse genetics
文献类型: 外文期刊
第一作者: Gao, Li
作者: Gao, Li;Qi, Xiaole;Li, Kai;Gao, Honglei;Gao, Yulong;Qin, Liting;Wang, Yongqiang;Wang, Xiaomei
作者机构:
关键词: vvIBDV;Tailored vaccine;Reverse genetics
期刊名称:VACCINE ( 影响因子:3.641; 五年影响因子:3.816 )
ISSN: 0264-410X
年卷期: 2011 年 29 卷 33 期
页码:
收录情况: SCI
摘要: Due to the problems associated with traditional methods for infectious bursal disease virus (IBDV) vaccine development and the pressure of evolution and variation of very virulent strains, it is urgent to develop IBDV vaccine rapidly with novel approaches. Using reverse genetics, the aim of this study was to generate a tailored vaccine strain (rGtHLJVP2) with its VP2 gene similar to very virulent IBDV (vvIBDV) to prevent the prevalence of IBDV. Characteristics of rGtHLJVP2 were evaluated in both cell culture and SPF chickens. rGtHLJVP2 replicated well as its parental strain Gt in vitro and in vivo. Immunization of SPF chickens with rGtHLJVP2 resulted in comparable antibody titers against IBDV as that of the medium virulent live vaccine B87, which was significant higher than that of attenuated vaccine Gt. Challenge studies with 10(4) ELD50 of prevalent homogeneous or heterogenous wIBDV revealed complete (100%) protection in the groups immunized with rGtHLJVP2. No significant clinical and pathological lesions were observed in chickens immunized with rGtHLJVP2. Our data demonstrated that rGtHLJVP2 could be used as a novel vaccine candidate for prevention against vvIBDV. (C) 2011 Published by Elsevier Ltd.
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