Delta-6 desaturase from borage converts linoleic acid to gamma-linolenic acid in HEK293 cells
文献类型: 外文期刊
第一作者: Chen, Qing
作者: Chen, Qing;Nimal, Jonathan;Li, Wanli;Liu, Xia;Cao, Wenguang
作者机构:
关键词: Borage;Delta 6-desaturase;gamma-Linolenic acid;Linoleic acid;HEK293 cell
期刊名称:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS ( 影响因子:3.575; 五年影响因子:3.381 )
ISSN: 0006-291X
年卷期: 2011 年 410 卷 3 期
页码:
收录情况: SCI
摘要: Gamma-linolenic acid (GLA, 18:3 n6) is an essential polyunsaturated fatty acid of the omega-6 family and is found to be effective in prevention and/or treatment of various health problems. In this study, we evaluated the possibility of increasing gamma-linolenic acid contents in mammalian cells using the delta-6 gene from Borago officinalis. The borage Delta 6-desaturase gene (sDelta-6) was codon-optimized and introduced into HEK293 cells by lipofectin transfection. Co-expression of GFP with sDelta-6 and RT-PCR analysis indicated that sDelta-6 could be expressed in mammalian cells. Subsequently, the heterologous expression of borage Delta 6-desaturase was evaluated by fatty acid analysis. Total cellular lipid analysis of transformed cells fed with linoleic acid (LA 18:2 n6) as a substrate showed that the expression of sDelta-6 resulted in an 228-483% (p < 0.05) increase of GLA when compared with that in the control cells. The highest conversion efficiency of LA into GLA in sDelta-6(+) cells was 6.9 times higher than that in the control group (11.59% vs. 1.69%; p < 0.05). Our present work demonstrated that the sDelta-6 gene from borage could be functionally expressed in mammalian cells, and could convert LA into GLA. Furthermore, this study may pave the way to generate transgenic livestock that can synthesise GLA. (C) 2011 Elsevier Inc. All rights reserved.
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