Development of an antigen-capture ELISA for the detection of equine influenza virus nucleoprotein

文献类型： 外文期刊

第一作者： Ji, Yuanyuan

作者： Ji, Yuanyuan;Guo, Wei;Zhao, Liping;Li, Hongmei;Lu, Gang;Wang, Zheng;Wang, Guibin;Liu, Cuiyun;Xiang, Wenhua

作者机构：

关键词： Equine influenza virus;Antigen-capture ELISA;Monoclonal antibody;Polyclonal antibody;Nucleoprotein

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2011 年 175 卷 1 期

页码：

收录情况： SCI

摘要： An antigen-capture enzyme-linked immunosorbent assay (AC-ELISA) was developed for the detection of the equine influenza virus (DV), employing monoclonal and polyclonal antibodies against the A/equine/Xingjiang/2007 (H3N8) nucleoprotein (NP). Immunoglobulin G antibodies were purified and used as capture or detector antibodies. The specificity of the optimized AC-ELISA was evaluated using EN, equine herpesvirus 1 (EHV-1), equine herpesvirus 4 (EHV-4), equine arteritis virus (EAV) and Japanese encephalitis virus UEV), resulting in only EIV specimens yielding a strong signal. A minimal concentration of 50 ng/ml EIV protein was detected in Nonidet P40-treated virus preparations. Virus from the nasal swabs of equines infected experimentally were detected from days 3 to 7 post-infection using this AC-ELISA, with results confirmed by virus isolation and multi reverse transcription polymerase chain reaction. Both H3N8 and H7N7 EIV subtypes were AC-ELISA positive, indicating that this assay is suitable for the detection of all EN subtypes. (C) 2011 Elsevier B.V. All rights reserved.

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