Identification of CD8(+) cytotoxic T lymphocyte epitopes from porcine reproductive and respiratory syndrome virus matrix protein in BALB/c mice

文献类型： 外文期刊

第一作者： Zhang, Weijun

作者： Zhang, Weijun;Lin, Yan;Bai, Yu;Tong, Tiegang;Wang, Qun;Liu, Nihong;Liu, Guangliang;Xiao, Yihong;Yang, Tao;Bu, Zhigao;Wu, Donglai;Tong, Guangzhi;Zhang, Weijun;Lin, Yan;Bai, Yu;Tong, Tiegang;Wang, Qun;Liu, Nihong;Liu, Guangliang;Xiao, Yihong;Yang, Tao;Bu, Zhigao;Tong, Guangzhi;Wu, Donglai

作者机构：

关键词： Porcine reproductive and respiratory syndrome virus;Matrix protein;CTL epitopes;Intracellular cytokine staining;ELISPOT

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2011 年 8 卷

页码：

收录情况： SCI

摘要： Twenty-seven nanopeptides derived from the matrix (M) protein of porcine reproductive and respiratory syndrome virus (PRRSV) were screened for their ability to elicit a recall interferon-gamma (IFN-gamma) response from the splenocytes of BALB/c mice following DNA vaccination and a booster vaccination with recombinant vaccinia virus rWR-PRRSV-M. We identified two peptides (amino acid residues K93FITSRCRL and F(57)GYMTFVHF) as CD8(+) cytotoxic T lymphocyte (CTL) epitopes. These peptides elicited significant numbers of IFN-gamma secreting cells, compared with other M nonapeptides and one irrelevant nonapeptide. Bioinformatics analysis showed that the former is an H-2K(d)-restricted CTL epitope, and the latter is an H-2D(d)-restricted CTL epitope. Multiple amino acid sequence alignment among different PRRSV M sequences submitted to GenBank indicated that these two CTL epitopes are strongly conserved, and they should therefore be considered for further research on the mechanisms of cellular immune responses to PRRSV.

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