Enhancement of bacteriolysis of Shuffled phage PhiX174 gene E

文献类型： 外文期刊

第一作者： Yu, Shen-ye

作者： Yu, Shen-ye;Peng, Wei;Si, Wei;Yin, Lu;Liu, Si-guo;Liu, Hui-fang;Zhao, Hai-ling;Wang, Chun-lai;Chang, Yue-hong;Lin, Yue-zhi

作者机构：

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2011 年 8 卷

页码：

收录情况： SCI

摘要： Bacterial ghosts that are generated using the regulated PhiX174 lysis gene E offer a new avenue for the study of inactivated vaccines. Here, we constructed a library of mutant gene E using a gene-shuffling technique. After screening and recombination with the prokaryotic non-fusion expression vector pBV220, two lysis plasmids were selected. Among which, a novel mutant E gene (named mE), consisting of a 74-bp non-encoding sequence at 5'-end and a 201-bp gene Delta E, significantly increased the lysis effect on prokaryotic Escherichia coli and Salmonella enteritidis. Moreover, lysis efficiency, as measured by the OD(600) value, reached 1.0 (10(9) CFU), avoiding the bottleneck problem observed with other bacterial lysis procedures, which results in a low concentration of bacteria in suspension, and consequent low production of bacterial ghosts. Our results may provide a promising avenue for the development of bacterial ghost vaccines.

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