Development of a reverse transcription loop-mediated isothermal amplification assay for detection of Porcine teschovirus

文献类型： 外文期刊

第一作者： Wang, Bin

作者： Wang, Bin;Tong, Guang-Zhi;Wang, Bin;Wang, Yongqiang;Tian, Zhi-Jun;An, Tong-Qing;Peng, Jin-Mei;Wang, Bin;Peng, Jin-Mei;Tong, Guang-Zhi

作者机构：

关键词： Diagnosis;Porcine teschovirus;reverse transcription loop-mediated isothermal amplification

期刊名称：JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION （ 影响因子：1.279； 五年影响因子：1.522 ）

ISSN： 1040-6387

年卷期： 2011 年 23 卷 3 期

页码：

收录情况： SCI

摘要： Loop-mediated isothermal amplification (LAMP) is a sensitive method for DNA amplification. In the present report, the development of a single-tube, one-step, real-time accelerated reverse transcription (RT)-LAMP for the detection of Porcine teschovirus (PTV) is described. Six designed primers amplified target gene sequences successfully at constant temperature (65 degrees C) within 1 hr, and the amplification results could be visualized directly by the naked eye. The sensitivity of the LAMP was 10 times higher than that of conventional polymerase chain reaction, and no cross-reactivity was found when the genomes of other common swine pathogens were subjected to the RT-LAMP system. When 43 clinical samples were tested by the RT-LAMP method, results indicated that the test is simple, rapid, accurate, and sensitive for the detection of PTV.

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