Salmonella enteritidis ghost vaccine induces effective protection against lethal challenge in specific-pathogen-free chicks

文献类型： 外文期刊

第一作者： Peng, Wei

作者： Peng, Wei;Si, Wei;Yin, Lu;Liu, Huifang;Yu, Shenye;Liu, Siguo;Wang, Chunlai;Chang, Yuehong;Zhang, Zhuo;Hu, Shouping;Du, Yanfen

作者机构：

关键词： Salmonella enteritidis;Ghost vaccine;Humoral and cellular immunity;Immune protection

期刊名称：IMMUNOBIOLOGY （ 影响因子：3.144； 五年影响因子：3.18 ）

ISSN： 0171-2985

年卷期： 2011 年 216 卷 5 期

页码：

收录情况： SCI

摘要： Bacterial ghosts (BGs) are empty bacterial envelopes generated by expulsion of the bacterial genome and cytoplasmic contents from bacterial cells, and the process is mediated by lysis protein E encoded on bacteriophage PhiX174. BGs represent a new approach in vaccine development and have been applied to a variety of gram-negative bacterial vaccine candidates. In this study, a BG vaccine generated from Salmonella enteritidis (S. enteritidis) strain DH091 was prepared using the highly efficient plasmid, pBV-mE. The efficacy of the BC vaccine was tested using 75 chicks (Gallus gallus) kept under specific pathogen-free (SPF) conditions. A comprehensive evaluation of the immune response, including humoral and cellular immune responses, interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) production, and histopathology of various tissues, was performed in BC-vaccinated animals subsequently challenged with S. enteritidis. The results were compared with animals that were immunized with the inactivated vaccine. S. enteritidis ghosts not only promoted the generation of high titer antibodies and IFN-gamma and IL-4 production but also stimulated a significant increase in CD8(+) and CD4(+) T lymphocytes. In particular, the dramatic increase in CD8(+) T cells indicated that the vaccine was able to induce clearance of intracellular Salmonella. The protective effects of BC vaccination in SPF chicks against 5 x 10(9) colony forming units of S. enteritidis were a result of the induction of a more effective immune response than that observed with the inactivated vaccine. These findings demonstrate the potential of S. enteritidis ghosts to be used as effective vaccines. (C) 2010 Elsevier GmbH. All rights reserved.

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