Cryopreservation of in vitro-grown apical meristems of Lilium by droplet-vitrification

文献类型： 外文期刊

第一作者： Chen, X. -L.

作者： Chen, X. -L.;Li, J. -H.;Xin, X.;Zhang, Z. -E.;Xin, P. -P.;Lu, X. -X.

作者机构：

关键词： Apical meristem;Cryopreservation;Droplet-vitrification method;Lily (Lilium L);Orthogonal experimental design

期刊名称：SOUTH AFRICAN JOURNAL OF BOTANY （ 影响因子：2.315； 五年影响因子：2.71 ）

ISSN： 0254-6299

年卷期： 2011 年 77 卷 2 期

页码：

收录情况： SCI

摘要： In this study, apical meristems from adventitious buds of three lily (Lillium L.) cultivars were successfully cryopreserved by droplet-vitrification. The most effective techniques were as follows. Excised meristems from in vitro plantlets which had been sub-cultured for about 2 months were cold-hardened at 4 degrees C for I week, precultured on MS medium supplemented with 0.3 mol L(-1) sucrose for 2 days, osmoprotected in loading solution for 20-40 min at room temperature and then soaked in PVS2 solution for 90-120 min at 0 degrees C, frozen in microdroplets of vitrification solution placed on aluminium foils, which were immersed rapidly in liquid nitrogen. The meristems were then rapidly rewarmed by dilution solution, transferred to regeneration medium and stored in the dark for two weeks at 20 degrees C, and then cultured under white fluorescent light at an intensity of 2000 lux, with a 16 h photoperiod at 20 degrees C. The highest post-thaw survival percentages of three cultivars 'Siberia' (Lilium x siberia), Lilium lancifolium Thunb. and 'Snow Queen' Lilium x longiflorum were 65.0%, 83.8% and 43.3%, and regeneration percentages were 62.0%, 67.6% and 35.0%, respectively. The study demonstrated that cryopreservation by droplet-vitrification increased survival and regeneration percentages of certain lily cultivars compared with vitrification. Thus to cryopreserve lily meristems, droplet-vitrification method is preferable to the vitrification method. Crown Copyright (C) 2010 Published by Elsevier B.V. on behalf of SAAB. All rights reserved.

分类号：

  • 相关文献

[1]STUDY ON THE EXTRACTION AND OXIDATION OF BIOACTIVE PEPTIDE FROM THE Sphauercerpus grailis. Leng, Fei-Fan,Zhu, Xin-Qiang,Wang, Xiao-Li,Leng, Fei-Fan,Sun, Shang-Chen,Liu, Lin,Liu, Xiaofeng,Wang, Yong-Gang.

[2]Cryopreservation of kiwi shoot tips. Wu, YJ,Zhao, YH,Engelmann, R,Zhou, MD. 2001

[3]Genetic stability assessments of plantlets regenerated from cryopreserved in vitro cultured grape and kiwi shoot-tips using RAPD. Zhai, ZY,Wu, YJ,Engelmann, F,Chen, RZ,Zhao, YH. 2003

[4]Cryopreservation of sea perch (Lateolabrax japonicus) spermatozoa and feasibility for production-scale fertilization. Ji, XS,Chen, SL,Tian, YS,Yu, GC,Sha, ZX,Xu, MY,Zhang, SC. 2004

[5]Optimization of Culture and Cryopreservation of Hand-Made Clone (HMC) Buffalo (Bubalus bubalis) Embryos. Yang, Chunyan,Pang, Chunying,Zheng, Haiying,Huang, Fenxiang,Wang, Jian,Shang, Jianghua,Yang, Bingzhuang,Liang, Xianwei. 2013

[6]Cryopreservation of apple shoot tips: Importance of cryopreservation technique and of conditioning of donor plants. Wu, YJ,Engelmann, F,Zhao, YH,Zhou, MD,Chen, SY. 1999

[7]Cryopreservation of Citrus anthers in the National Crop Genebank of China. Zhang, Jin-Mei,Lu, Xin-Xiong,Xin, Xia,Yin, Guang-Kun,He, Juan-Juan,Huang, Bin,Chen, Xiao-Ling,Huang, Bin,Jiang, Dong. 2017

[8]Evaluating the Impacts of Osmotic and Oxidative Stress on Common Carp (Cyprinus carpio, L.) Sperm Caused by Cryopreservation Techniques. Li, Ping,Li, Zhi-Hua,Dzyuba, Boris,Hulak, Martin,Rodina, Marek,Linhart, Otomar,Li, Ping,Li, Zhi-Hua,Dzyuba, Boris. 2010

[9]PLANT-REGENERATION FROM RICE (ORYZA-SATIVA L.) EMBRYOGENIC SUSPENSION CELLS CRYOPRESERVED BY VITRIFICATION. HUANG, CN,WANG, JH,YAN, QS,ZHANG, XQ,YAN, QF. 1995

[10]Cryopreservation of the sperm of spotted halibut Verasper variegates (Pleuronectiformes, Pleuronectidae). Liu, XZ,Zhang, SC,Zhang, YZ,Xu, YJ. 2006

[11]Birth of calves expressing the enhanced green fluorescent protein after transfer of fresh or vitrified/thawed blastocysts produced by somatic cell nuclear transfer. Gong, GC,Dai, YP,Fan, BL,Zhu, HB,Zhu, S,Wang, HP,Wang, LL,Tang, B,Li, R,Wan, R,Liu, Y,Huang, YH,Zhang, L,Sun, XZ,Li, N.

[12]Improvement of Agrobacterium-mediated transformation efficiency and transgenic plant regeneration of Vitis vinifera L. by optimizing selection regimes and utilizing cryopreserved cell suspensions. Li, P,Hanania, U,Sahar, N,Mawassi, M,Gafny, R,Sela, I,Tanne, E,Perl, A.

[13]Establishment and biological characteristics of Ujumqin sheep fibroblast line. Na, Ri Su,Zhao, Qian Jun,Jin, Da Peng,Su, Xiao Hua,Chen, Xian Wei,Guan, Wei Jun,Ma, Yue Hui,Jin, Da Peng,Su, Xiao Hua,Chen, Xian Wei.

[14]Transcriptome analysis and identification of induced genes in the response of Harmonia axyridis to cold hardiness. Tang, Bin,Liu, Xiao-Jun,Shi, Zuo-Kun,Shen, Qi-Da,Xu, Yan-Xia,Wang, Shi-Gui,Wang, Su,Zhang, Fan.

[15]Cryopreservation of in vitro cultured shoot tips of strawberry. Zhao, Mizhen,Wang, Zhuangwei,Su, Jiale,Qian, Yaming. 2006

[16]Sperm of the giant grouper: cryopreservation, physiological and morphological analysis and application in hybridizations with red-spotted grouper. Tian, Yongsheng,Jiang, Jing,Wang, Na,Qi, Wenshan,Liang, You,Chen, Songlin,Jiang, Jing,Qi, Wenshan,Yang, Chuanjun,Zhai, Jieming,Li, Bo,Chen, Youming.

[17]Cryopreservation and multipotential characteristics evaluation of a novel type of mesenchymal stem cells derived from Small Tailed Han Sheep fetal lung tissue. Ma, Caiyun,Liu, Changqing,Li, Xiangchen,Bai, Chunyu,Fan, Yanan,Guan, Weijun,Guo, Yu,Liu, Changqing,Guo, Yu,Lu, Taofeng.

[18]Cryopreservation of common carp (Cyprinus carpio L.) sperm induces protein phosphorylation in tyrosine and threonine residues. Li, P.,Hulak, M.,Li, Z. H.,Psenicka, M.,Rodina, M.,Gela, D.,Linhart, O.,Li, P.,Li, Z. H.,Sulc, M..

[19]Establishment and genetic characteristics analysis of in vitro culture a fibroblast cell line derived from Wuzhishan miniature pig. Liu, Changqing,Guo, Yu,Liu, Changqing,Lu, Taofeng,Li, Xiangchen,Guan, Weijun,Ma, Yuihui.

[20]Cryopreservation of flounder (Paralichthys olivaceus) sperm with a practical methodology. Zhang, YZ,Zhang, SC,Liu, XZ,Xu, YY,Wang, CL,Sawant, MS,Li, J,Chen, SL.

作者其他论文 更多>>

微信小程序