Fungistatic Intensity of Agricultural Soil Against Fungal Agents and Phylogenetic Analysis on the Actinobacteria Involved

文献类型: 外文期刊

第一作者: Fang, Li Zhi

作者: Fang, Li Zhi;Kun, Xu Chuan;Qun, Duan Chang;He, Mo Ming;Fang, Li Zhi;Kun, Xu Chuan;Qun, Duan Chang;He, Mo Ming;Fang, Li Zhi;Song, Zou Chang;Qin, Xi Jia;Qiu, He Yue

作者机构:

期刊名称:CURRENT MICROBIOLOGY ( 影响因子:2.188; 五年影响因子:2.197 )

ISSN: 0343-8651

年卷期: 2011 年 62 卷 4 期

页码:

收录情况: SCI

摘要: A total of 287 agricultural soil samples collected from 26 provinces or autonomous regions of China were tested on their ability to suppress the conidial germination of nine biocontrol fungal agents. These soil samples showed great differences in the degree to inhibit the germination of conidia (22.8% 50%) to most of tested fungi. Ten soil samples that have stronger fungistatic intensity (germination inhibition rate >68.3%) to the target fungi, Trichoderma viride and Paecilomyces lilacinus, were selected to evaluate their soil actinobacteria involved fungistasis in soil. Of the 1,000 isolates from those soil samples, 345 actinobacteria exhibited fungistatic activity to conidial germination of T. viride and P. lilacinus with germination inhibition rates higher than 10%. Sequences encoding 16S rRNA gene of the 345 actinobacteria were analyzed by ARDRA and resulted 44 different ARDRA types. Fifty-six isolates, at least one from each unique ARDRA type, were selected for 16S rDNA sequencing and phylogenetic analysis. Results indicated that the actinobacteria involved in the soil fungistasis had close phylogenetic relationship with the members of Sterptomycetaceae, Microbacteriaceae, Micrococcaceae, and Nocardiacea.

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