A laccase with antiproliferative activity against tumor cells from an edible mushroom, white common Agrocybe cylindracea

文献类型： 外文期刊

第一作者： Hu, D. D.

作者： Hu, D. D.;Zhang, G. Q.;Wang, H. X.;Hu, D. D.;Zhang, G. Q.;Wang, H. X.;Zhang, R. Y.;Ng, T. B.

作者机构：

关键词： Agrocybe cylindracea;Antiproliferative activity;Edible mushroom;HIV-1 reverse transcriptase;Laccase

期刊名称：PHYTOMEDICINE （ 影响因子：5.34； 五年影响因子：5.161 ）

ISSN： 0944-7113

年卷期： 2011 年 18 卷 5 期

页码：

收录情况： SCI

摘要： A laccase, with HIV-1 reverse transcriptase inhibitory activity (IC50 = 12.7 mu M) and antiproliferative activity against HepG2 cells (IC50 = 5.6 mu M) and MCF7 cells (IC50 = 6.5 mu M), was purified from fresh fruiting bodies of the edible white common Agrocybe cylindracea mushroom. The laccase, which had a novel N-terminal sequence, displayed a molecular mass of 58 kDa within the range reported for most other mushroom laccases. The purification protocol entailed ion exchange chromatography on DEAE-cellulose, SP-Sepharose, and Q-Sepharose and gel filtration on Superdex 75. The laccase was adsorbed on DEAE-cellulose and Q-Sepharose, but unadsorbed on SP-Sepharose. Its optimum pH was pH 3-4 and its optimum temperature was 50 degrees C. The activity of the isolated laccase differed from one substrate to another. The ranking was ABTS > N,N-dimethyl-1,4-phenylenediamine > hydroquinone > catechol > 2-methylcatechol > pyrogallol. (C) 2010 Elsevier GmbH. All rights reserved.

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