Cloning, codon-optimized expression and homology modeling of structural protein VP1 from foot and mouth disease virus

文献类型： 外文期刊

第一作者： Liu, Xin-sheng

作者： Liu, Xin-sheng;Wang, Yong-lu;Zhang, Yong-guang;Fang, Yu-zhen;Pan, Li;Lue, Jian-liang;Zhou, Peng;Zhang, Zhong-wang;Cheng Qi-wei;Wang, Gang;Wang, Ji-wei;Lou, Hui;Jiang, Shou-tian

作者机构：

关键词： Foot-and-mouth disease virus;VP1 protein;codon optimization;homology modeling

期刊名称：AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH （ 影响因子：0.539； 五年影响因子：0.564 ）

ISSN： 1996-0808

年卷期： 2011 年 5 卷 5 期

页码：

收录情况： SCI

摘要： Structural protein VP1 of foot-and-mouth disease virus ( FMDV) is the most frequently studied protein due to its significant roles in virus attachment, protective immunity, and serotype specificity. The coding sequence of VP1was amplified and then identified by polymerase chain reaction (PCR) and sequencing. To achieve high-level expression of VP1 protein, we optimized VP1 gene base on Escherichia coli preferred codons and synthesized the optimized gene. The synthetical gene was cloned into the fusion expression vector pET-28a and expressed in E. coli BL21(DE3). After induced with Isopropyl beta-D-1-Thiogalactopyranoside (IPTG) and optimized the conditions of expression, the VP1 fusion protein was highly expressed and identified in inclusion bodies by SDS-PAGE and Western blotting. Based on the primary and secondary structure analysis of VP1, Three-dimensional structure of VP1 was developed by homology modeling methods. The validation of 3-D structure was done with the help of PROCHECK encompassing amino acid residues in the most favored region of almost all strains. Potential epitopes of VP1 was predicted with different methods. In this study, the VP1 protein was expressed in E. coli efficiently and highly purified VP1 was obtained, which laid a foundation of refolding and further study on activity of the protein. The VP1 model in the productive conformation can now be used for structure-based design purposes as well as structure-function relation of VP1 protein.

分类号：

  • 相关文献

[1]Foot-and-mouth disease virus VP1 protein fused with cholera toxin B subunit expressed in Chlamydomonas reinhardtii chloroplast. Sun, M,Qian, KX,Su, N,Chang, HY,Liu, JX,Chen, GF.

[2]Integrity of foot-and-mouth disease virions bound to the cells. Yi, J.,Liu, Ch.. 2010

[3]Prokaryote expression of HPeV-1 VP1 protein, production of VP1 polyclonal antibody and the development of an ELISA. Yu, Ying,Shan, Tongling,Zhu, Caixia,Hua, Xiuguo,Shan, Tongling,Wang, Chunmei,Zhu, Shaofeng,Wang, Chunmei.

[4]Evidence of VP1 of duck hepatitis A type 1 virus as a target of neutralizing antibodies and involving receptor-binding activity. Li, Xiaojun,Zhao, Ran,Li, Chenxi,Zhang, Tingting,Meng, Fanyi,Liu, Ming,Zhang, Yun,Lin, Wei.

[5]Characterization of monoclonal antibodies against duck hepatitis type 1 virus VP1 protein. Zhang, Tingting,Li, Xiaojun,Wu, Xiaoying,Shaozhou, Wulin,Bai, Xiaofei,Liu, Siguo,Liu, Ming,Zhang, Yun.

[6]High expression of foot-and-mouth disease virus structural protein VP1 in tobacco chloroplasts. Li, Y,Sun, M,Liu, JX,Yang, ZQ,Zhang, ZF,Shen, GF. 2006

[7]Recombinant Bivalent Vaccine against Foot-and-Mouth Disease Virus Serotype O/A Infection in Guinea Pig. Yi, JZ,Liu, MQ,Zhu, CZ,Zhang, Q,Sheng, ZT,Du, QY,Yan, WY,Zheng, ZX.

[8]Genetic characterization of duck hepatitis a viruses isolated in China. Li, Jing,Bi, Yuhai,Chen, Can,Yang, Limin,Liu, Wenjun,Liu, Wenjun,Ding, Chan.

[9]Functional analyses of the chitin-binding domains and the catalytic domain of Brassica juncea chitinase BjCHI1. Tang, CM,Chye, ML,Ramalingam, S,Ouyang, SW,Zhao, KJ,Ubhayasekera, W,Mowbray, SL. 2004

[10]Molecular characterization and expression analysis of beta(2)-microglobulin in large yellow croaker Pseudosciaena crocea. Yu, Suhong,Chen, Xinhua,Ao, Jingqun,Yu, Suhong,Yu, Suhong. 2009

[11]Allelic variation in TLR4 is linked to resistance to Salmonella Enteritidis infection in chickens. Zhao, Xingwang,Liu, Ranran,Song, Yongmei,Li, Qinghe,Zheng, Maiqing,Cui, Huanxian,Zhao, Guiping,Wen, Jie,Li, Peng,Everaert, Nadia,Wang, Huihua,Gou, Zhongyong.

[12]Exploring the Molecular Basis for Binding of Inhibitors by Threonyl-tRNA Synthetase from Brucella abortus: A Virtual Screening Study. Li, Ming,Wen, Fang,Zhao, Shengguo,Wang, Pengpeng,Li, Songli,Zhang, Yangdong,Zheng, Nan,Wang, Jiaqi,Li, Ming,Wen, Fang,Zhao, Shengguo,Wang, Pengpeng,Li, Songli,Zhang, Yangdong,Zheng, Nan,Wang, Jiaqi,Zheng, Nan,Wang, Jiaqi. 2016

[13]New mutations existing in the nucleocapsid (N) gene of non-porcine TGEV strains isolated in China. Yu, Xiaolong,Man, Chaolai. 2011

[14]Phylogenetic and structural analysis of major surface proteins hemagglutinin and neuraminidase of novel avian influenza virus A H7N9 from chinese patient. Yuan Xiao-hui,Wang Ying-chen,Qu Zhang-yi,Yuan Xiao-hui,Ren Jia-yi,Yuan Xiao-hui,Ren Jia-yi,Wu Xiao-min,Wang Jing-fei. 2013

[15]Novel Rapid Molecular Modeling Method Based on Evolutional Tree for Human Adenovirus Hexon Proteins Family. Yuan Xiao-Hui,Wang Ying-Chen,Qu Zhang-Yi,Gao Hong,Yuan Xiao-Hui,Ren Jia-Yi,Yuan Xiao-Hui,Ren Jia-Yi,Ren Jia-Yi,Wang Ya-Xian,Hua Dong,Wang Jing-Fei,Guo Ying-Ying,Wu Xiao-Min,Yang Zhi-Wei. 2011

[16]Construction of multiple recombinant SLA-I proteins by linking heavy chains and light chains in vitro and analyzing their secondary and 3-dimensional structures. Gao, Feng-shan,Bai, Jing,Xu, Chong-bo,Zhang, Qiang,Li, Yanmin.

[17]New insights into substrate folding preference of plant OSCs. Ma, Yongshuo,Zhou, Yuan,Huang, Sanwen,Shang, Yi,Ovchinnikov, Sergey,Greisen, Per, Jr.,Greisen, Per, Jr.,Huang, Sanwen,Shang, Yi. 2016

[18]Characterization of a new cry2Ab gene of Bacillus thuringiensis with high insecticidal activity against Plutella xylostella L.. Pan, Zhizhen,Zhu, Yujing,Shi, Huai,Chen, Zheng,Chen, Meichun,Liu, Bo,Xu, Lian,Chen, Qingxi.

[19]Expression, purification and molecular characterization of a novel endoglucanase protein from Bacillus subtilis SB13. Guan, Xuefang,Chen, Penglian,Xu, Qingxian,Qian, Lei,Huang, Juqing,Lin, Bin.

[20]Non-uniqueness of factors constraint on the codon usage in Bombyx mori. Jia, Xian,Zheng, Hao,Qi, Qi,Wei, Lei,Sun, Jingchen,Jia, Xian,Zheng, Hao,Qi, Qi,Wei, Lei,Sun, Jingchen,Liu, Shuyu,He, Jian,Li, Bo,Zhao, Taiyi. 2015

作者其他论文 更多>>

微信小程序