Isolation and analysis of ZmPto from maize, a homologue to Pto

文献类型： 外文期刊

第一作者： Zou, Huawen

作者： Zou, Huawen;Song, Zhongjian;Wu, Zhongyi;Zhang, Xiuhai;Huang, Conglin;Zou, Huawen;Liu, Hongfang;Ma, Guohui

作者机构：

关键词： Zea mays L.;ZmPti1;Abiotic stress;Salicylic acid;Yeast two-hybrid

期刊名称：PLANT OMICS （ 影响因子：0.777； 五年影响因子：0.802 ）

ISSN： 1836-0661

年卷期： 2011 年 4 卷 2 期

页码：

收录情况： SCI

摘要： Pto has been reported to play important roles in plant disease responses. The cloning and characterization of the Pto-like gene named as ZmPto from maize was reported in this research. The open reading frame sequence of ZmPto was obtained by using RT-PCR. Sequence analysis showed that ZmPto encodes a polypeptide of 405 amino acids with predicted molecular mass of 45.0 kDa and pI of 6.01. Yeast two-hybrid analysis showed the interaction of ZmPto with ZmPti1. RT-PCR analysis indicated that the ZmPto expression is induced by salicylic acid (SA), abscisic acid (ABA), mannitol and salt suggesting that ZmPto may play important roles in both biotic and abiotic signal transduction pathway. Additionally, the ZmPto displays different expression patterns in different tissues indicating its multiple roles in plant.

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