An acid and highly thermostable xylanase from Phialophora sp G5

文献类型： 外文期刊

第一作者： Zhang, Fan

作者： Zhang, Fan;Shi, Pengjun;Bai, Yingguo;Luo, Huiying;Yuan, Tiezheng;Huang, Huoqing;Yang, Peilong;Yao, Bin;Zhang, Fan;Miao, Lihong

作者机构：

关键词： Phialophora sp G5;Xylanase;Thermostability;Broad substrate specificity

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2011 年 89 卷 6 期

页码：

收录情况： SCI

摘要： An endo-beta-1,4-xylanase gene, designated xyn10G5, was cloned from Phialophora sp. G5 and expressed in Pichia pastoris. The 1,197-bp full-length gene encodes a polypeptide of 399 amino acids consisting of a putative signal peptide at residues 1-20, a family 10 glycoside hydrolase domain, a short Gly/Thr-rich linker and a family 1 carbohydrate-binding module (CBM). The deduced amino acid sequence of XYN10G5 shares the highest identity (53.4%) with a putative xylanase precursor from Aspergillus terreus NIH2624. The purified recombinant XYN10G5 exhibited the optimal activity at pH 4.0 and 70 degrees C, remained stable at pH 3.0-9.0 (>70% of the maximal activity), and was highly thermostable at 70 degrees C (retaining similar to 90% of the initial activity for 1 h). Substrate specificity studies have shown that XYN10G5 had the highest activity on soluble wheat arabinoxylan (350.6 U mg(-1)), and moderate activity to various heteroxylans, and low activity on different types of cellulosic substrates. Under simulated gastric conditions, XYN10G5 was stable and released more reducing sugars from soluble wheat arabinoxylan; when combined with a glucanase (CelA4), the viscosity of barley-soybean feed was significantly reduced. These favorable enzymatic properties make XYN10G5 a good candidate for application in the animal feed industry.

分类号：

  • 相关文献

[1]Two neutral thermostable cellulases from Phialophora sp G5 act synergistically in the hydrolysis of filter paper. Zhao, Junqi,Shi, Pengjun,Li, Zhongyuan,Yang, Peilong,Luo, Huiying,Bai, Yingguo,Wang, Yaru,Yao, Bin. 2012

[2]A novel thermoacidophilic and thermostable endo-beta-1,4-glucanase from Phialophora sp G5: its thermostability influenced by a distinct beta-sheet and the carbohydrate-binding module. Zhao, Junqi,Shi, Pengjun,Huang, Huoqing,Li, Zhongyuan,Yuan, Tiezheng,Yang, Peilong,Luo, Huiying,Bai, Yingguo,Yao, Bin. 2012

[3]A Thermophilic Cellulase Complex from Phialophora sp G5 Showing High Capacity in Cellulose Hydrolysis. Zhao, Junqi,Shi, Pengjun,Bai, Yingguo,Huang, Huoqing,Luo, Huiying,Xu, Donghao,Wang, Yaru,Yao, Bin,Zhang, Huitu. 2012

[4]The disruption of two salt bridges of the cold-active xylanase XynGR40 results in an increase in activity, but a decrease in thermostability. Wang, Guozeng,Wu, Jingjing,Lin, Juan,Ye, Xiuyun,Yao, Bin.

[5]A novel, alkali-tolerant thermostable xylanase from Saccharomonospora viridis: direct gene cloning, expression and enzyme characterization. Wang, Ziyuan,Jin, Yi,Wu, Huijun,Xie, Xiangming,Tian, Zhaofeng,Wu, Yuying.

[6]Improvement of alkali stability and thermostability of Paenibacillus campinasensis Family-11 xylanase by directed evolution and site-directed mutagenesis. Zheng, Hongchen,Liu, Yihan,Han, Yang,Lu, Fuping,Zheng, Hongchen,Liu, Yihan,Sun, Mingzhe,Han, Yang,Wang, Jianling,Lu, Fuping,Zheng, Hongchen,Sun, Junshe,Liu, Yihan,Wang, Jianling,Sun, Mingzhe,Lu, Fuping.

[7]Gene cloning, expression, and characterization of a thermostable xylanase from Nesterenkonia xinjiangensis CCTCC AA001025. Kui, Hong,Luo, Huiying,Shi, Pengjun,Bai, Yingguo,Yuan, Tiezheng,Wang, Yaru,Yang, Peilong,Yao, Bin,Kui, Hong,Dong, Shouliang.

[8]A thermophilic endo-1,4-beta-glucanase from Talaromyces emersonii CBS394.64 with broad substrate specificity and great application potentials. Wang, Kun,Luo, Huiying,Bai, Yingguo,Shi, Pengjun,Huang, Huoqing,Xue, Xianli,Yao, Bin. 2014

[9]A thermophilic alpha-galactosidase from Neosartorya fischeri P1 with high specific activity, broad substrate specificity and significant hydrolysis ability of soymilk. Wang, Huimin,Shi, Pengjun,Luo, Huiying,Huang, Huoqing,Yang, Peilong,Yao, Bin. 2014

[10]Purification, gene cloning and characterization of an acidic beta-1,4-glucanase from Phialophora sp G5 with potential applications in the brewing and feed industries. Zhao, Junqi,Shi, Pengjun,Yuan, Tiezheng,Huang, Huoqing,Li, Zhongyuan,Meng, Kun,Yang, Peilong,Yao, Bin. 2012

[11]Molecular Cloning and Expression of a Novel beta-Glucosidase Gene from Phialophora sp G5. Li, Xuejun,Zhao, Junqi,Shi, Pengjun,Yang, Peilong,Wang, Yaru,Luo, Huiying,Yao, Bin,Li, Xuejun. 2013

[12]A two-step discriminated method to identify thermophilic proteins. Tang, Hua,Cao, Ren-Zhi,Wang, Wen,Liu, Tie-Shan,Wang, Li-Ming,He, Chun-Mei. 2017

[13]Purification and characterization of a psychrophilic catalase from Antarctic Bacillus. Wang, Wei,Sun, Mi,Zhang, Bin,Liu, Wanshun. 2008

[14]An intramolecular disulfide bond is required for the thermostability of methyl parathion hydrolase, OPHC2. Chu, Xiao-yu,Tian, Jian,Wu, Ning-feng,Fan, Yun-liu.

[15]Heterologous expression of a gene encoding a thermostable beta-galactosidase from Alicyclobacillus acidocaldarius. Yuan, Tiezheng,Yang, Peilong,Wang, Yaru,Meng, Kun,Luo, Huiying,Yao, Bin,Zhang, Wei,Wu, Ningfeng,Fan, Yunliu.

[16]Structure-Based Design of Mucor pusillus Pepsin for the Improved Ratio of Clotting Activity/Proteolytic Activity in Cheese Manufacture. Zhang, Jie,Sun, Yonghai,Luo, Quan,Zhang, Jie,Li, Zhuolin,Li, Tiezhu,Wang, Tuoyi.

[17]Genetic and biochemical characterization of a protease-resistant mesophilic beta-mannanase from Streptomyces sp S27. Shi, Pengjun,Yuan, Tiezheng,Zhao, Junqi,Huang, Huoqing,Luo, Huiying,Meng, Kun,Wang, Yaru,Yao, Bin.

[18]IMPROVEMENT ON THE THERMOSTABILITY AND ACTIVITY OF APX1 FROM ENERGY PLANT JATROPHA CURCAS L. BY HYPER-ACIDIC FUSION PARTNERS. Zhang, Mengru,Yang, Yumei,Li, Xujuan,Wang, Haibo,Yang, Shuanglong,Wang, Shasha,Gong, Ming,Zou, Zhurong,Li, Xujuan,Wang, Haibo. 2014

[19]A novel thermophilic endo-beta-1,4-mannanase from Aspergillus nidulans XZ3: functional roles of carbohydrate-binding module and Thr/Ser-rich linker region. Lu, Haiqiang,Luo, Huiying,Shi, Pengjun,Huang, Huoqing,Meng, Kun,Yang, Peilong,Yao, Bin. 2014

[20]Structural Insights into the Thermophilic Adaption Mechanism of Endo-1,4-beta-Xylanase from Caldicellulosiruptor owensensis. Liu, Xin,Xin, Fengjiao,Wen, Boting,Gu, Tianyi,Wang, Fengzhong,Sun, Lichao,Liu, Tengfei,Shi, Xinyuan,Zhang, Yuebin,Mi, Shuofu. 2018

作者其他论文 更多>>

微信小程序