The plant alkaloid piperine as a potential inhibitor of ethidium bromide efflux in Mycobacterium smegmatis

文献类型： 外文期刊

第一作者： Jin, Jing

作者： Jin, Jing;Feng, Haihua;Li, Lei;Liang, Junchao;Wu, Xiuping;Wang, Xuelin;Liu, Mingyuan;Deng, Xuming;Yu, Lu;Zhang, Jiyu;Guo, Na;Sun, Kai

作者机构：

期刊名称：JOURNAL OF MEDICAL MICROBIOLOGY （ 影响因子：2.472； 五年影响因子：2.836 ）

ISSN： 0022-2615

年卷期： 2011 年 60 卷 2 期

页码：

收录情况： SCI

摘要： Piperine, a major plant alkaloid found in black pepper (Piper nigrum) and long pepper (Piper Ion gum), has shown potential for inhibiting the efflux pump (EP) of Staphylococcus aureus. In this study, a modulation assay showed that piperine could decrease the MIC of ethidium bromide (EtBr) twofold at 32 mu g ml(-1) and fourfold at 64 mu g ml(-1) against Mycobacterium smegmatis mc(2) 155 ATCC 700084. A real-time, 96-well plate fluorometric method was employed to evaluate the EP inhibition ability of piperine in M. smegmatis. Reserpine, chlorpromazine, verapamil and carbonyl cyanide m-chlorophenylhydrazone were used as positive controls. Piperine significantly enhanced accumulation and decreased the efflux of EtBr in M. smegmatis, which suggests that it has the ability to inhibit mycobacterial EPs.

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