Determination of Urea in Milk and Milk Products by In-line Dialysis-Flow Injection-Spectrophotometry

文献类型： 外文期刊

第一作者： Zhao Li-Jing

作者： Zhao Li-Jing;Yan Jie;Ma Dong-Sheng;Xiao Jing-Ze;Zhao Ping;Fan Zhi-Ying

作者机构：

关键词： Flow injection;In-line dialysis;Urea;Milk

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2011 年 39 卷 2 期

页码：

收录情况： SCI

摘要： An in-line dialysis flow-injection spectrophotometric method was developed to determine urea in milk and milk products. Protein was precipitated from a sample portion by the addition of trichloroacetic acid solution. The precipitated milk protein was removed by centrifuging and filtration. Remaining protein and fat which could interfere with the subsequent analysis was removed by in-line dialysis. The spectrophotometric method was based on the reaction of urea with diacetyl which was produced from diacetylmonoxime hydrolysis in acid solution when the Fe3+ co-existed in solution. The red compound obtained from the said reaction was measured at 534 nm. To enhance the intensity of the color, aminothiourea was added. Experimental parameters, including the reagent concentration, on-line dialysis unit structure, the length of the mixing coil, were optimized. Under optimized conditions,the linear calibration curves were obtained for the concentration range of 0 - 500. 0 mg/kg (r >= 0. 999). The proposed Method had low detection limit of 1. 3 mg/kg urea. The relative standard deviation was 0. 9% - 2. 0% with the sample throughput of 22 samples/h. The urea was determined various milk products with the standard addition recovery of 94. 1% - 109. 8%. The method was simple, rapid and has been used to determine the urea in milk and milk products with satisfactory results.

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