Loop-mediated isothermal amplification assay targeting the ompA gene for rapid detection of Riemerella anatipestifer

文献类型： 外文期刊

第一作者： Zheng, Fuying

作者： Zheng, Fuying;Lin, Guozhen;Zhou, Jizhang;Wang, Guanghua;Cao, Xiaoan;Gong, Xiaowei;Qiu, Changqing

作者机构：

关键词： LAMP;ompA gene;PCR;Riemerella anatipestifer

期刊名称：MOLECULAR AND CELLULAR PROBES （ 影响因子：2.365； 五年影响因子：2.386 ）

ISSN： 0890-8508

年卷期： 2011 年 25 卷 1 期

页码：

收录情况： SCI

摘要： A novel loop-mediated isothermal amplification (LAMP) assay was developed and evaluated for the detection of Riemerella anatipestifer (RA) infection. The LAMP assay exhibited a higher sensitivity than conventional polymerase chain reaction (PCR) and microbial isolation. The specificity of the assay was determined by restriction enzyme digestion of the LAMP products and detection of Escherichia coli, Salmonella enterica and Pasteurella multocida. The LAMP assay was able to detect RA effectively in samples of the reference strains, isolated strains and infected duck brains. This assay is a useful tool for the diagnosis of RA infection in the clinical setting. (C) 2010 Elsevier Ltd. All rights reserved.

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