Primary purification of two antifungal proteins from leaves of the fig (Ficus carica L.)

文献类型： 外文期刊

第一作者： Pan, Ying-hong

作者： Pan, Ying-hong;Zhao, Ming;Ma, Yan;Yuan, Wen-xia;Yan, Wei

作者机构：

关键词： Fig;antifungal protein;chromatography;natural food preservative

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2011 年 10 卷 3 期

页码：

收录情况： SCI

摘要： Low-molecular-weight extracts of fig (Ficus carica L.) leaves has antifungal and antibacterial activities against several types of microorganisms. In this work, two high-molecular-weight fractions with antifungal activity, termed figinI and figinII were obtained from leaves of fig using a procedure including ion-exchange chromatography (SP-Sepharose Fast Flow), hydrophobic-interaction chromatography (Phenyl Sepharose 6 Fast Flow and RESOURCE ISO) and ion-exchange chromatography (Mono S). By matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis (MALDI-TOF MS), the molecular mass of figinI was 21531Da and figinII was 31957Da. This is the first report on isolation of antifungal proteins from F. carica L., and it shows their potential for further investigation.

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