ASSOCIATION BETWEEN POLYMORPHISMS IN THE SLC27A1 GENE AND MILK PRODUCTION TRAITS IN CHINESE HOLSTEIN CATTLE

文献类型： 外文期刊

第一作者： Lv, Yanfei

作者： Lv, Yanfei;Wei, Caihong;Zhang, Li;Lu, Guobin;Du, Lixin;Lv, Yanfei;Liu, Kaidong

作者机构：

关键词： Chinese Holstein cattle;Milk production traits;PCR-RFLP;PCR-SSCP;SLC27A1 gene

期刊名称：ANIMAL BIOTECHNOLOGY （ 影响因子：2.282； 五年影响因子：1.702 ）

ISSN： 1049-5398

年卷期： 2011 年 22 卷 1 期

页码：

收录情况： SCI

摘要： We assessed SLC27A1, a candidate gene for milk production traits in Chinese Holstein cattle. DNA was extracted from the blood of 48 top Chinese Holstein Cattle selected according to phenotypic character and mixed into DNA pool for SNP detection. We tested blood samples of these cattle for SNPs in exon 3 and the 3'-flanking region of the SLC27A1 gene by using polymerase chain reaction-single-stranded conformation polymorphism (PCR-SSCP) and DNA sequencing. We found 2 polymorphic sites: 112T>C, a synonymous mutation, in exon 3 (SNP1), and 64G>A in the 3'-UTR (SNP2). We also determined the genotypes of 330 Chinese Holstein cattle by using PCR-restriction fragment length polymorphism (RFLP). We found 3 genotypes each at SNP1 (TT, TC, and CC) and SNP2 (GG, GA, and AA). The association among the different genotypes at these 2 sites and milk production traits was analyzed using a least-squares procedure. The results showed that cows with genotype CC had higher milk yields than those with genotype TC (0.01

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