Expression and antigenicity characterization for truncated capsid protein of porcine circovirus type 2

文献类型： 外文期刊

第一作者： Lou, Zhongzi

作者： Lou, Zhongzi;Li, Xuerui;Li, Zhiyong;Yin, Xiangping;Li, Baoyu;Lan, Xi;Yang, Bin;Zhang, Yun;Liu, Jixing

作者机构：

期刊名称：CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE （ 影响因子：1.31； 五年影响因子：1.438 ）

ISSN： 0830-9000

年卷期： 2011 年 75 卷 1 期

页码：

收录情况： SCI

摘要： Three pairs of specific primers were designed to amplify F2-1, F2-2, and XF2-2 truncated capsid protein genes of porcine circovirus type 2 (PCV-2). Amplified sequences were subcloned to pET-32a(1) vectors and expressed in Rosetta (DE3) Escherichia coli by induction of isopropy-beta-D-thiogalactoside (IPTG). All of the fusion proteins had positive reactions to PCV-2 antiserum and His-XF2-2 showed the best reactivity. Proteins were used to immunize BALB/c mice to produce monoclonal antibodies (mAbs), and 7 mAbs were selected. Capsid protein N-terminal parts 55 to 96 amino acid (aa), 97 to 141 aa, and 143 to 211 aa were confirmed as binding regions of the 7 mAbs. Reactivity between His-XF2-2 and the 7 mAbs was detected, FmAb-8 showed the best reactivity. The dominant B-cell epitope was located at 97 to 141 aa. The PEPSCAN indicated that the P122-136 peptide contained the dominant B-cell epitope.

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