Temporal and Spatial Expression of the Pax-7 Gene During Chicken Embryo and Postnatal Development

文献类型： 外文期刊

第一作者： Chang Guobin

作者： Chang Guobin;Liao Jing;Chen Rong;Luan Deqin;Zhang Ying;Ma Teng;Zhou Wei;Chen Guohong;Liu Xiangping;Dai Aiqing;Wang Kehua

作者机构：

关键词： Chicken;pax-7 gene;RT-PCR;spatiotemporal expression;tissue;China

期刊名称：JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.365； 五年影响因子：0.359 ）

ISSN： 1680-5593

年卷期： 2011 年 10 卷 14 期

页码：

收录情况： SCI

摘要： This study was conducted to investigate the different temporal and spatial expression pattern of pax-7 gene in embryo stage and growth stage of chicken, aiming to explore associations between muscle development and molecular markers of pax-7 gene. Breast and leg muscle tissue were selected from 8, 16, 20 and 21 days chick embryo and 2, 4, 6, 8, 10, 12 weeks rugao chickens. This study performed SYBR Green real-time fluorescence quantitative PCR to detect the expression pattern of pax-7 gene. The results showed that the expression pattern of pax-7 gene was similar between breast and leg muscles which it is lower during chicken embryo and reached to the highest point at 8-10 weeks of age. Pax-7 gene was continual expressed during chicken embryo and postnatal development, not only expressed in the embryo stage. Fin. ally, we reassess current models of early patterning based on the analysis of these dynamic spatiotemporal expression patterns.

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