A reverse-transcription, loop-mediated isothermal amplification assay for detection of bovine ephemeral fever virus in the blood of infected cattle

文献类型： 外文期刊

第一作者： Zheng, Fuying

作者： Zheng, Fuying;Lin, Guozhen;Zhou, Jizhang;Wang, Guanghua;Cao, Xiaoan;Gong, Xiaowei;Qiu, Changqing

作者机构：

关键词： Bovine ephemeral fever virus;RT-LAMP;RT-PCR;Virus isolation

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2011 年 171 卷 1 期

页码：

收录情况： SCI

摘要： A novel reverse-transcription, loop-mediated isothermal amplification (RT-LAMP) assay for the detection of bovine ephemeral fever virus (BEFV) was developed and evaluated in this study. The RT-LAMP assay exhibited higher sensitivity when compared with conventional reverse-transcription polymerase chain reaction (RT-PCR) and virus isolation methods. The specificity of the assay was determined by digestion of the RT-LAMP products with restriction enzyme and detection of BEFV serogroup rabies virus (RV). Using RT-LAMP, RT-PCR and virus isolation methods, 36 blood samples were tested and the results indicated that RT-LAMP could detect early infection with BEFV. The RT-LAMP method is useful for the diagnosis of BEFV infection in blood samples. (C) 2010 Published by Elsevier B.V.

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