OsRab5a regulates endomembrane organization and storage protein trafficking in rice endosperm cells

文献类型： 外文期刊

第一作者： Wang, Yihua

作者： Wang, Yihua;Ren, Yulong;Liu, Xi;Jiang, Ling;Chen, Liangming;Han, Xiaohua;Liu, Shijia;Liu, Feng;Lv, Jia;Zhou, Kunneng;Wan, Jianmin;Jin, Mingna;Su, Ning;Wan, Jianmin;Bao, Yiqun

作者机构：

关键词： OsRab5a;glutelin;57H mutant;endosperm cells;protein trafficking;endomembrane organization

期刊名称：PLANT JOURNAL （ 影响因子：6.417； 五年影响因子：7.627 ）

ISSN： 0960-7412

年卷期： 2010 年 64 卷 5 期

页码：

收录情况： SCI

摘要： P>Rice glutelins are synthesized at the endoplasmic reticulum (ER) as precursors (pro-glutelins), and are transported to protein storage vacuoles, where they are processed into mature proteins. The molecular basis of this process is largely unknown. Here, we report the isolation of a rice mutant, gpa1, that accumulates 57 kDa pro-glutelins in seeds and whose endosperm has a floury appearance. Transmission electron microscopy analysis showed that the gpa1 endosperm cells have an enlarged ER lumen and a smaller protein body II (PBII), and accumulated three types of newly generated subcellular structures. Moreover, a proportion of glutelins in the gpa1 endosperm cells were not delivered to PBII, and instead were mis-targeted to two of the newly generated structures or secreted. The gene corresponding to the gpa1 mutation was found to be OsRab5a, which encodes a small GTPase. In Arabidopsis protoplasts, OsRab5a protein was found to co-localize predominantly with AtVSR2, a molecular marker for the pre-vacuolar compartments (PVC). We conclude that OsRab5a plays an essential role in trafficking of storage protein to PBII, possibly as part of its function in organizing the endomembrane system in developing endosperm cells of rice.

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