OsLB2.2 negatively regulates rice disease resistance at seedling stage in rice

文献类型： 外文期刊

第一作者： Ma, Tianze

作者： Ma, Tianze;Wang, Mengge;Xie, Shupeng;Wang, Jingguo;Liu, Hualong;Yang, Luomiao;Zou, Detang;Zheng, Hongliang;Xin, Wei;Xie, Shupeng;Men, Longnan;Sun, Zhonghua;Zong, Tianpeng;Li, Chengxin;Xin, Wei

作者机构：

关键词： rice; blast; genome-wide association study; RNA-seq; resistance gene

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：4.8； 五年影响因子：5.7 ）

ISSN： 1664-462X

年卷期： 2025 年 16 卷

页码：

收录情况： SCI

摘要： Background Rice blast disease, caused by the fungal pathogen Magnaporthe oryzae, stands as the most destructive diseaset of rice, negatively impacting yield and quality. Identification of blast resistance genes is crucial for breeding disease resistant varieties. In this study, we conducted a genome-wide association analysis on rice blast with 295 Japonica rice varieties and the rice blast dominant physiological race ZD5 from Heilongjiang.Results A total of 11 Quantitative Trait Loci (QTLs) encompassing 233 genes were mapped. Notably, 40 genes showed significant phenotypic variations among different haplotypes. By combining GWAS and RNA sequencing analysis, we identified five candidate genes related to rice seedling resistance to rice blast. Through the assessment of relative gene expression levels in 10 susceptible and 10 resistant varieties, OsLB2.2 (LOC_Os02g57470) emerged as a key gene displaying significant expression differences in resistant varieties under uninoculated and inoculated conditions. To elucidate gene functionality, we generated oslb2.2 knockout mutants. The results showed that oslb2.2 knockout mutants were significantly enhanced rice blast resistance compared to the wild type. Furthermore, we identified the dominant haplotype of OsLB2.2 and developed Kompetitive Allele - Specific PCR (KASP) molecular markers for molecular improvement of rice blast resistance.Conclusion The phenotypic validation indicated that OsLB2.2 negatively regulated the rice blast resistance at the seedling stage. Moreover, KASP molecular markers were developed, providing a theoretical basis for the potential application of OsLB2.2 in molecular breeding strategies.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序