Development of new microsatellite markers for molecular diversity analysis of Citrus species
文献类型: 外文期刊
第一作者: Dixit, A.
作者: Dixit, A.;Chung, J. W.;Zhao, W. G.;Kim, C. H.;Park, Y. J.;Kim, C. H.;Park, Y. J.;Zhao, W. G.;Lee, G. A.;Ma, K. H.;Lee, M. C.;Gwag, J. G.;Lee, D. H.;Dixit, A.
作者机构:
期刊名称:JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY ( 影响因子:1.641; 五年影响因子:1.616 )
ISSN: 1462-0316
年卷期: 2010 年 85 卷 6 期
页码:
收录情况: SCI
摘要: Thirty microsatellite loci for further genetic analysis of Citrus species were developed by constructing a microsatellite-enriched library using capture with streptavidin-coated magnetic beads and used to assess genetic diversity in 40 Citrus accessions. In total. 150 alleles were detected, with an average of five alleles per locus. The average gene diversity and polymorphism information content values were 0.58 and 0.52, and ranged from 0.35 - 0.74 and from 0.32 - 0.70, respectively. Values for the observed and expected heterozygosities ranged from 0.13 - 1.00 and from 0.36 - 0.75, respectively. Fifteen loci deviated from the Hardy-Weinberg equilibrium (P < 0.05). The mean similarity coefficient among accessions was 0.5906. Based on the UPGMA algorithm, two main groups were successfully identified. These new microsatellite markers can be used to further investigate the genetics of, and phylogenic relationships in Citrus spp.
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