Farnesol, a Potential Efflux Pump Inhibitor in Mycobacterium smegmatis

文献类型: 外文期刊

第一作者: Jin, Jing

作者: Jin, Jing;Li, Lei;Liang, Jun-Chao;Wang, Xue-Lin;Li, Yang;Liu, Ming-Yuan;Wu, Xiu-Ping;Yu, Lu;Zhang, Ji-Yu;Guo, Na;Sheng, Hui

作者机构:

关键词: farnesol;inhibitor;efflux pump;Mycobacterium smegmatis

期刊名称:MOLECULES ( 影响因子:4.411; 五年影响因子:4.587 )

ISSN: 1420-3049

年卷期: 2010 年 15 卷 11 期

页码:

收录情况: SCI

摘要: The active multidrug efflux pump (EP) has been described as one of the mechanisms involved in the natural drug resistance of bacteria, such as mycobacteria. As a result, the development of efflux pumps inhibitors (EPIs) is an important topic. In this study, a checkerboard synergy assay indicated that farnesol both decreased the minimum inhibitory concentration (MIC) of ethidium bromide (EtBr) 8-fold against Mycobacterium smegmatis (M. smegmatis) mc(2)155 ATCC 700084 when incorporated at a concentration of 32 mu g/mL (FICI = 0.625) and decreased MIC 4-fold at 16 mu g/mL (FICI = 0.375). Farnesol also showed synergism when combined with rifampicin. A real-time 96-well plate fluorometric method was used to assess the ability of farnesol to inhibit EPs in comparison withfour positive EPIs: chlorpromazine, reserpine, verapamil, and carbonyl cyanide m-chlorophenylhydrazone (CCCP). Farnesol significantly enhanced the accumulation of EtBr and decreased the efflux of EtBr in M. smegmatis; these results suggest that farnesol acts as an inhibitor of mycobacterial efflux pumps.

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