Construction of mini-Tn4001 transposon vector for Mycoplasma gallisepticum

文献类型： 外文期刊

第一作者： Chen HongJun

作者： Chen HongJun;Zhao ChunMei;Shen XieYue;Chen DanQing;Yu ShengQing;Ding Chan

作者机构：

关键词： Mycoplasma gallisepticum;mini-Tn4001 transposon vector;mycoplasma transformation

期刊名称：SCIENCE CHINA-LIFE SCIENCES （ 影响因子：6.038； 五年影响因子：4.754 ）

ISSN： 1674-7305

年卷期： 2010 年 53 卷 11 期

页码：

收录情况： SCI

摘要： The detailed genetic analysis of mycoplasmas has long been hampered by the lack of appropriate tools for genetic manipulation. In this study, the transposon vector, mini-Tn4001tetM, was constructed containing the tnp gene, encoding a transposase gene in Staphylococcus aureus, two copies of the IS256 inverted repeat sequence (inner and outer) and the tetM gene, from the Enterococcus faecalis Tn916 transposon, conferring resistance to tetracycline. This vector was electro-transformed into Mycoplasma gallisepticum (MG). The recombinant cells were screened by tetracycline selection. The results indicated that the transposon vector could replicate in MG strain R by successive passages, indicating that MG is a potential vector for expressing protective antigens of other pathogens.

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