Deletion of 1.8-kb mRNA of Marek's disease virus decreases its replication ability but not oncogenicity

文献类型： 外文期刊

第一作者： Ding, Jiabo

作者： Ding, Jiabo;Sun, Aijun;Wang, Jingyan;Su, Shuai;Cui, Zhizhong;Li, Yanpeng;Zhu, Hongfei;Chen, Hongjun

作者机构：

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2010 年 7 卷

页码：

收录情况： SCI

摘要： Background: The 1.8-kb mRNA was reported as one of the oncogenesis-related genes of Marek's disease virus (MDV). In this study, the bacterial artificial chromosome (BAC) clone of a MDV field strain GX0101 was used as the platform to generate mutant MDV to examine the functional roles of 1.8-kb mRNA. Results: Based on the BAC clone of GX0101, the 1.8-kb mRNA deletion mutant GX0101 Delta(A+C) was constructed. The present experiments indicated that GX0101 Delta(A+C) retained a low level of oncogenicity, and it showed a decreased replication capacity in vitro and in vivo when compared with its parent virus, GX0101. Further studies in vitro demonstrated that deletion of 1.8-kb mRNA significantly decreased the transcriptional activity of the bidirectional promoter between 1.8-kb mRNA and pp38 genes of MDV. Conclusion: These results suggested that the 1.8-kb mRNA did not directly influence the oncogenesis but related to the replication ability of MDV.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序