Construction of Neospora caninum stably expressing TgSAG1 and evaluation of its protective effects against Toxoplasma gondii infection in mice
文献类型: 外文期刊
第一作者: Zhang, Guohong
作者: Zhang, Guohong;Huang, Xiaohong;Boldbaatar, Damdinsuren;Battur, Banzragch;Battsetseg, Badgar;Zhang, Houshuang;Yu, Longzheng;Li, Yan;Luo, Yuzi;Cao, Shinuo;Goo, Youn-Kyong;Yamagishi, Junya;Zhou, Jinlin;Zhang, Shoufa;Suzuki, Hiroshi;Igarashi, Ikuo;Mikami, Takeshi;Nishikawa, Yoshifumi;Xuan, Xuenan;Huang, Xiaohong;Boldbaatar, Damdinsuren;Battur, Banzragch;Battsetseg, Badgar;Zhang, Houshuang;Zhou, Jinlin;Zhang, Shoufa
作者机构:
关键词: Neospora caninum;Toxoplasma gondii;TgSAG1;Recombinant vaccine;Vector
期刊名称:VACCINE ( 影响因子:3.641; 五年影响因子:3.816 )
ISSN: 0264-410X
年卷期: 2010 年 28 卷 45 期
页码:
收录情况: SCI
摘要: Toxoplasma gondii and Neospora caninum are closely related apicomplexan parasites. The surface antigen 1 of T. gondii (TgSAG1) is a major immunodominant antigen and, therefore, is considered to be a good candidate for the development of an effective recombinant vaccine against toxoplasmosis. In this study, N. caninum stably expressing the TgSAG1 gene (Nc/TgSAG1) was constructed using pyrimethamine-resistant DHFR-TS and GFP genes as double-selection markers. The expression level, molecular weight, and antigenic property of recombinant TgSAG1 expressed by the Nc/TgSAG1 were similar to those of the native TgSAG1. The mice immunized with Nc/TgSAG1 induced TgSAG1-specific Th1-dominant immune responses and protected the mice from a lethal challenge infection with T. gondii. These results indicate that N. caninum may provide a new tool for the production of a live recombinant vector vaccine against toxoplasmosis in animals. To our knowledge, this is the first report to evaluate the usefulness of N. caninum-based live vaccine. (C) 2010 Elsevier Ltd. All rights reserved.
分类号:
- 相关文献
作者其他论文 更多>>
-
Integration of transcriptome, histopathology, and physiological indicators reveals regulatory mechanisms of largemouth bass ( Micropterus salmoides) in response to carbonate alkalinity stress
作者:Hua, Jixiang;Xi, Bingwen;Qiang, Jun;Hua, Jixiang;Tao, Yifan;Lu, Siqi;Li, Yan;Dong, Yalun;Jiang, Bingjie;Xi, Bingwen;Qiang, Jun
关键词:Micropterus salmoides; Carbonate alkalinity stress; Tissue damage; Serum biological chemistry; RNA-seq
-
Identification of the MAP4K gene family reveals GhMAP4K13 regulates drought and salt stress tolerance in cotton
作者:Zeng, Qing;Wang, Junjuan;Wang, Shuai;Lu, Xuke;Li, Yan;Ye, Wuwei;Yin, Zujun;Peng, Fanjia;Bakhsh, Allah;Qaraevna, Bobokhonova Zebinisso;Ye, Wuwei;Yin, Zujun
关键词:
-
Effect of combined nitrogen and phosphorus fertilization on summer maize yield and soil fertility in coastal saline-alkali land
作者:Ma, Changjian;Wang, Yue;Liu, Lining;Wang, Xuejun;Sun, Zeqiang;Li, Yan;Ma, Changjian;Wang, Yue;Wu, Wenbiao;Hou, Peng;Li, Bowen;Yuan, Huabin
关键词:Grain yield; Biomass yield; Fertilizer physiological efficiency; Coastal saline-alkali land
-
Comparative genomic analysis reveals the difference of NLR immune receptors between anthracnose-resistant and susceptible sorghum cultivars
作者:Zhang, Ji-Wei;Li, Jin-Yang;Yu, Zhi-Fan;Chang, Xin-Ya;Han, Jun-Ru;Xia, Jing-Yang;Kami, Yam Bahadur;Wang, He;Li, Yan;Wang, Wen-Ming;Sun, Yuan-Tao;Ni, Xian-Lin;Li, Ling;Wang, Song-Tao
关键词:Sorghum; Anthracnose; NLR receptor; Colletotrichum sublineola; Genetic variation; Differential gene expression
-
The role of the nitrate transporter NRT1.1 in plant iron homeostasis and toxicity on ammonium
作者:Li, Guangjie;Zhang, Lin;Wang, Yanqin;Li, Yan;Wang, Zhaoyue;Shi, Weiming;Kronzucker, Herbert J.;Kronzucker, Herbert J.;Chen, Gui
关键词:Ammonium toxicity; Iron; Nitrate; NRT1.1; Root growth
-
Context-dependent response of crop pests to landscape composition
作者:Yang, Long;Pan, Yunfei;Wyckhuys, Kris A. G.;Li, Minlong;Wang, Kaitao;Liu, Bing;Liu, Yangtian;Jia, Shuangshuang;Li, Qian;Li, Yan;Lu, Yanhui;Wyckhuys, Kris A. G.;Desneux, Nicolas
关键词:Agroecology; context dependency; ecological based pest management; ecological intensification; host quality
-
TaSnRK3.23B, a CBL-interacting protein kinase of wheat, confers drought stress tolerance by promoting ROS scavenging in Arabidopsis
作者:Dong, Feiyan;Liu, Yide;Zhang, Huadong;Li, Yaqian;Chen, Sheng;Wang, Shuailei;Zhu, Zhanwang;Liu, Yike;Song, Jinghan;Li, Yan
关键词:Wheat;
TaSnRK3.23B ; Ectopic expression; Drought stress; CBL proteins