Construction of Neospora caninum stably expressing TgSAG1 and evaluation of its protective effects against Toxoplasma gondii infection in mice

文献类型: 外文期刊

第一作者: Zhang, Guohong

作者: Zhang, Guohong;Huang, Xiaohong;Boldbaatar, Damdinsuren;Battur, Banzragch;Battsetseg, Badgar;Zhang, Houshuang;Yu, Longzheng;Li, Yan;Luo, Yuzi;Cao, Shinuo;Goo, Youn-Kyong;Yamagishi, Junya;Zhou, Jinlin;Zhang, Shoufa;Suzuki, Hiroshi;Igarashi, Ikuo;Mikami, Takeshi;Nishikawa, Yoshifumi;Xuan, Xuenan;Huang, Xiaohong;Boldbaatar, Damdinsuren;Battur, Banzragch;Battsetseg, Badgar;Zhang, Houshuang;Zhou, Jinlin;Zhang, Shoufa

作者机构:

关键词: Neospora caninum;Toxoplasma gondii;TgSAG1;Recombinant vaccine;Vector

期刊名称:VACCINE ( 影响因子:3.641; 五年影响因子:3.816 )

ISSN: 0264-410X

年卷期: 2010 年 28 卷 45 期

页码:

收录情况: SCI

摘要: Toxoplasma gondii and Neospora caninum are closely related apicomplexan parasites. The surface antigen 1 of T. gondii (TgSAG1) is a major immunodominant antigen and, therefore, is considered to be a good candidate for the development of an effective recombinant vaccine against toxoplasmosis. In this study, N. caninum stably expressing the TgSAG1 gene (Nc/TgSAG1) was constructed using pyrimethamine-resistant DHFR-TS and GFP genes as double-selection markers. The expression level, molecular weight, and antigenic property of recombinant TgSAG1 expressed by the Nc/TgSAG1 were similar to those of the native TgSAG1. The mice immunized with Nc/TgSAG1 induced TgSAG1-specific Th1-dominant immune responses and protected the mice from a lethal challenge infection with T. gondii. These results indicate that N. caninum may provide a new tool for the production of a live recombinant vector vaccine against toxoplasmosis in animals. To our knowledge, this is the first report to evaluate the usefulness of N. caninum-based live vaccine. (C) 2010 Elsevier Ltd. All rights reserved.

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