Insect-resistance and high-yield transgenic tobacco obtained by molecular breeding technology

文献类型: 外文期刊

第一作者: Wang Youru

作者: Wang Youru;Wang Youru;Guo Sandui

作者机构:

关键词: VHb gene;insectidal gene;transgenic plant;pest resistance;yield

期刊名称:AFRICAN JOURNAL OF BIOTECHNOLOGY ( 影响因子:0.573; 五年影响因子:0.794 )

ISSN: 1684-5315

年卷期: 2010 年 9 卷 40 期

页码:

收录情况: SCI

摘要: The modified synthesized VHb gene and insectidal gene (GFMcryIA) were transferred to tobacco plants by Agrobacterium-mediated transformation. The bivalent genes were inserted successfully into the tobacco genome and detected by PCR amplification. Southern blot and Western blot analyses showed that VHb gene was expressed in the transgenic plants. Toxicity assay indicated that insectidal gene expressed pesticidal toxin protein. The net weight of transgenic tobacco plants exceeded that of non-transgenic ones by 8%. Compared to non-transgenic tobacco plants, transgenic plants appeared to be high-yielding, insect-resistant varieties.

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