Production of xylooligosaccharides from the steam explosion liquor of corncobs coupled with enzymatic hydrolysis using a thermostable xylanase

文献类型： 外文期刊

第一作者： Teng, Chao

作者： Teng, Chao;Jiang, Zhengqiang;Fan, Guangsen;Yan, Qiaojuan;Shi, Bo

作者机构：

关键词： Corncobs;Enzymatic hydrolysis;Steam explosion;Steam explosion liquor of corncobs (SELC);Thermostable xylanase

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.642； 五年影响因子：9.237 ）

ISSN： 0960-8524

年卷期： 2010 年 101 卷 19 期

页码：

收录情况： SCI

摘要： The production of xylooligosaccharides (XOs) from corncobs was studied using a two-stage process based on a steam explosion pretreatment followed by enzymatic hydrolysis. Corncobs with different chip sizes were subjected to steam explosion under different experimental conditions of temperature and time, namely 188-204 degrees C for 2.5-7.5 min. The results indicate that corncobs were optimally steam exploded at 196 degrees C for 5 min, resulting in hemicellulose recovery of 22.8%. Especially, corncobs with large chip sizes (similar to 100 mm) during steam explosion pretreatment were suitable to produce XOs. Furthermore, a thermostable xylanase from Paecilomyces themophila J18 was used to hydrolyze steam explosion liquor of corncobs (SELC) for the production of XOs. A maximum XOs yield of 28.6 g XOs/100 g xylan in corncobs was achieved and XOs syrup contained more than 90% of xylobiose and xylotriose when the hydrolysis was carried out under the optimized conditions (pH 7.0, 70 degrees C, 7.5 U mL(-1) and 2.5 h). These results suggest that the process might be effective in production of XOs for industrial applications. (C) 2010 Elsevier Ltd. All rights reserved.

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