Development and evaluation of a VP1-ELISA for detection of antibodies to duck hepatitis type 1 virus
文献类型: 外文期刊
第一作者: Zhang, Tingting
作者: Zhang, Tingting;Zhang, Yun;Meng, Fanyi;Li, Xiaojun;Kong, Xiangang;Liu, Ming;Hou, Zhenzhong;Feng, Xinchang
作者机构:
关键词: Duck hepatitis type 1 virus (DHV-1);VP-1 protein;Neutralization test;Western blot;ELISA
期刊名称:JOURNAL OF VIROLOGICAL METHODS ( 影响因子:2.014; 五年影响因子:2.001 )
ISSN: 0166-0934
年卷期: 2010 年 169 卷 1 期
页码:
收录情况: SCI
摘要: The VP1-encoding gene of the duck hepatitis type 1 virus (DHV-1) HP-1 strain was cloned and expressed in Escherichia coli. The open reading frame (ORF) of VP1 comprised 714 bp and encoded 238 amino acids, with a predicated molecular mass of 26.5 kDa. The expressed VP1 fusion protein in E. coli was detected by Western blotting with duck anti-DHV-1 polyclonal serum. A VP1-ELISA using the expressed VP1 protein as a coating antigen for the detection of antibodies to DHV-1 in ducks was developed. In comparison with the virus neutralization test, the specificity and sensitivity of the VP1-ELISA was 92.5% and 96.7%. Comparative analysis between Western blots and the VP1-ELISA showed that the concordance between the two methods was 86%. The VP1-ELISA did not react with the anti-sera to other duck viral pathogens, implying that this protein is specific for the recognition of duck anti-DHV-1 antibodies. Taken together, the VP1-ELISA is a highly sensitive and specific test that could be used for screening for DHV-1 infection and monitoring antibody titres against DHV-1. (c) 2010 Elsevier B.V. All rights reserved.
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