Molecular characterization and expression analysis of porcine integrins alpha v beta 3, alpha v beta 6 and alpha v beta 8 that are potentially involved in FMDV infection

文献类型： 外文期刊

第一作者： Du, Junzheng

作者： Du, Junzheng;Chang, Huiyun;Gao, Shandian;Xue, Shuang;Cong, Guozheng;Shao, Junjun;Lin, Tong;Liu, Zaixin;Liu, Xiangtao;Cai, Xuepeng

作者机构：

关键词： FMDV receptors;Pig;Molecular characterization;Expression analysis;Tropism

期刊名称：MOLECULAR AND CELLULAR PROBES （ 影响因子：2.365； 五年影响因子：2.386 ）

ISSN： 0890-8508

年卷期： 2010 年 24 卷 5 期

页码：

收录情况： SCI

摘要： In the present study, we report the sequences and characterization of the porcine integrin cDNAs encoding alpha v, beta 3, beta 6 and beta 8 subunits and compare them to those of other species. The coding sequences for the porcine alpha v, beta 3, beta 6 and beta 8 subunits were found to be 3141, 2289, 2367 and 2304 nucleotides in length, encoding 1046, 762, 788 and 767-amino-acid-residue protein, respectively. The porcine integrin alpha v, beta 3, beta 6 and beta 8 subunit shares common structural and functional elements with their counterparts from the other species. Phylogenetic trees showed that the porcine alpha v, beta 3, beta 6 and beta 8 were clustered into the Artiodactyla group, together with those of camels, sheep, and cattle, that are susceptible to FMDV infection. Real-time RT-PCR was used to investigate expression of the integrins alpha v, beta 3, beta 6 and beta 8 in different tissues of pigs in order to determine the role of these receptors in tissue tropism. Expression analysis showed that alpha v beta 6 and alpha v beta 8 mRNA expression were detected at high levels in tissues known to support replication of FMDV. Tissue distribution pattern of alpha v beta 3 mRNA seems to be unrelated to the known tissue tropism of FMDV. This study provided the first data of porcine integrins for the further studies of the FMDV pathogenesis in pigs. (C) 2010 Elsevier Ltd. All rights reserved.

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