Computational identification and characteristics of novel microRNAs from the silkworm (Bombyx mori L.)

文献类型: 外文期刊

第一作者: Huang, Yong

作者: Huang, Yong;Tang, Shun Ming;Wang, Li Gang;Shen, Xing Jia;Zou, Quan;Huang, Yong;Tang, Shun Ming;Wang, Li Gang;Shen, Xing Jia

作者机构:

关键词: MicroRNA;Bombyx mori;Computational prediction;Target gene

期刊名称:MOLECULAR BIOLOGY REPORTS ( 影响因子:2.316; 五年影响因子:2.357 )

ISSN: 0301-4851

年卷期: 2010 年 37 卷 7 期

页码:

收录情况: SCI

摘要: MicroRNAs (miRNAs) are a class of non-protein coding small RNAs that regulate expression of genes at post-transcriptional levels. Increasing evidence has shown that miRNAs play multiple roles in biological processes, including development, cell proliferation and apoptosis. Based on the conservation of miRNAs sequence, using a computational homology search based on genomic survey sequence analysis, a total of 16 novel miRNAs were identified and characteristics such as family and evolutionary conservation have been described. By using these newly identified miRNAs, the mRNA database of silkworm was blasted and 21 potential targets of miRNAs were detected. Most of these miRNA targeted genes were predicted to encode transcription factors. The semi-stem loop RT-PCR based assays were performed and found that silkworm miRNAs have diverse expression patterns during development.

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