Molecular Cloning and cDNA Sequence Analysis of Two New Lepidopteran OR83b Orthologue Chemoreceptors

文献类型: 外文期刊

第一作者: Xiu Wei-ming

作者: Xiu Wei-ming;Dong Shuang-lin;Zhang Yi-fan;Liu Yu-sheng;Xiu Wei-ming;Zhang Yi-fan;Yang Dian-lin

作者机构:

关键词: Spodoptera exigua;Spodoptera litura;olfactory receptor gene;sequence analysis

期刊名称:AGRICULTURAL SCIENCES IN CHINA ( 影响因子:0.82; 五年影响因子:0.997 )

ISSN: 1671-2927

年卷期: 2010 年 9 卷 8 期

页码:

收录情况: SCI

摘要: The aim of this study was to isolate the full-length cDNA sequences of an unusually highly conserved olfactory receptor (orthologue to the Drosophila melanogaster DOR83b) from the antennae of Spodoptera exigua and S. litura by using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) methods. Bioinformatics methods were used to further analyze the cDNA sequences and putative amino acid sequences. The two full-length cDNA sequences from olfactory receptor (OR) of male S. exigua and S. litura were named as SexiOR2 and SlitOR2, respectively. SexiOR2 and SlitOR2 consisted of nucleotide sequence of 1 906 and 2 483 bp, respectively, and both with deduced amino acid sequences of 473 residues. The sequence analysis indicated that the deduced amino acid sequences of the cDNA shared the high homologies with OR83b orthologue chemoreceptor sequences from previously reported moths, implying that the cDNA sequences were of OR83b orthologue chemoreceptor genes.

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